柱前衍生化HPLC法测定注射用芪红脉通中氨基酸  

Determination of Amino Acids in Qihong Maitong Injection through Pre-column Derivatization HPLC

作　　者：赵祎武[1,2] 张宏达[1,2] 谢雪[1,2] 王伟[1,2] 洪奎[1,2] 黄文哲[1,2] 王振中[1,2] 萧伟[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,连云港222000 [2]中药制药过程新技术国家重点实验室,连云港222000

出　　处：《世界科学技术-中医药现代化》2015年第5期1080-1084,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科技部重大新药创制项目(2013ZX09402203):现代中药创新集群与数字制药技术平台;负责人:王振中

摘　　要：目的:建立中药注射剂注射用芪红脉通中氨基酸含量测定方法。方法:采用OPA-FMOC柱前衍生化法,用HPLC方法同时检测注射用芪红脉通中17种氨基酸。结果:所测17种氨基酸线性关系良好。结论:本试验建立的HPLC检测氨基酸的分析方法,选择性强、重复性好,可用于注射用芪红脉通中氨基酸的检测及质量控制。In this article, an HPLC method for the contents determination of amino acids in Qihong Maitong injection was reported. In detailed, OPA-Fmoc pre-column derivatization was adopted, and related HPLC methods to determine the contents of amino acids was established. Linear relationship was well constructed for 17 amino acids through the method mentioned above. Briefly speaking, the optimized method was accurate and reproducible, and suitable for the determination of amino acids in Qihong Maitong injection and corresponding quality control.

关 键 词：注射用芪红脉通 氨基酸 柱前衍生化法 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]