高效液相色谱法同时测定通脉冲剂中葛根素、阿魏酸和丹酚酸B的含量  

Determination of Puerarin,Ferulaic Acid and Salvianolic Acid B in Tongmai Medicinal Instant Granules by HPLC

作　　者：徐海燕 孔令提[2] 

机构地区：[1]重庆市南川区人民医院药剂科,重庆408400 [2]蚌埠医学院第一附属医院药剂科,安徽蚌埠233000

出　　处：《中国医院用药评价与分析》2015年第7期866-868,共3页Evaluation and Analysis of Drug-use in Hospitals of China

摘　　要：目的：采用高效液相色谱法同时测定通脉冲剂中葛根素、阿魏酸和丹酚酸B的含量。方法：色谱柱为岛津ODS-C18柱（250 mm×4.6 mm,5μm）,流动相为甲醇-0.1%甲酸（V∶V=30∶70）,流速为1.0 ml/min,柱温为35℃,检测波长为321 nm。结果：葛根素、阿魏酸和丹酚酸B的质量浓度分别在40.20-402.00、1.05-10.50、39.80-398.00μg/ml内与峰面积积分值呈良好线性关系,r均为0.999 9。三者精密度、稳定性、重复性试验的RSD均〈2%;三者平均加样回收率分别为100.20%、99.75%和99.81%,RSD分别为1.02%、1.34%和0.97%。结论：该法简便可行、重复性好,可用于控制通脉冲剂的质量。OBJECTIVE： To establish HPLC determination of puerarin,ferulaic acid and salvianolic acid B in Tongmai medicinal instant granules. METHODS： The chromatographic separation of the samples was performed on a C18column（ 250 mm × 4. 6 mm,5 μm） maintained at 35℃ and the mobile phase consisted of methanol（ A） and0. 1% formic acid（ B）（ V ∶ V = 30 ∶ 70） at a flow rate of 1. 0 ml / min and detective wavelength of 321 nm. RESULTS：The linear ranges of puerarin,ferulaic acid and salvianolic acid B were 40. 20-402. 00 μg / ml,1. 05-10. 50 μg / ml and39. 80-398. 00 μg / ml,respectively（ r = 0. 999 9）. The RSDs of the tests of precision,reproducibility and stability were all lower than 2%. The average recoveries were 100. 20%,99. 75% and 99. 81%,respectively with RSDs of1. 02%,1. 34% and 0. 97%,respectively. CONCLUSIONS： The method is simple and reproducible and it can beused for the quality control of Tongmai medicinal instant granules.

关 键 词：通脉冲剂 葛根素 阿魏酸 丹酚酸B 高效液相色谱法 

分 类 号：R965[医药卫生—药理学]