微泡超声空化声压幅度与大鼠血脑屏障通透性关系的研究  

Relationship of ultrasound cavitation pressure amplitude and rat blood brain barrier permeability

作　　者：崔海[1] 徐亚丽[1] 吴盛正[1] 李露[1] 高顺记[1] 刘政[1] 

机构地区：[1]第三军医大学新桥医院超声科,重庆400378

出　　处：《中华超声影像学杂志》2015年第8期715-719,共5页Chinese Journal of Ultrasonography

基　　金：国家自然科学基金（81227004,81471795）;重庆市自然科学基金（2010BB5186）

摘　　要：目的探讨不同峰值负压（声压）激励的微泡超声空化调控大鼠血脑屏障通透性的有效性。方法30只大鼠，依据声压值分为5组：62kPa组，86kPa组，181kPa组，324kPa组，708kPa组。静脉注入微泡，超声空化仪经颞骨辐照9min，以对侧大脑半球为对照组。采用伊文思蓝定量分析、镧剂示踪及病理组织学损伤评价。结果伊文思蓝定量值：181kPa组为（4．52±0．11）μg／g，708kPa组为（10．56±0．13）μg／g，组间差异均有统计学意义（P〈0．05）。镧剂示踪提示181kPa组表现为开放趋势，708kPa组呈增强开放，病理学观察后者可出现点状红细胞外漏，为可逆性损伤。结论微泡超声空化的声压调节可有效地调控开放大鼠血脑屏障。Objective To explore the effectiveness of different peak negative pressure ultrasound irradiated microbubble destruction on the control of rat blood brain barrier permeability. Methods Thirty healthy SD rats were divided into 5 groups according to different ultrasound pressure level （62 kPa,86 kPa, 181 kPa,324 kPa,708 kPa）. After intravenous injection of microbubble,the rats were underwent ultrasonie irradiation for 9 minutes, and the contralateral hemispheres were served as controls. The permeability of blood brain barrier was determined by the quantitative analysis of Evans blue and lanthanum nitrate extravasation. Results Quantitative analysis results revealed a leakage in Evans blue about（4.52 ± 0.11）μg/g and （10.56 ±0.13）μg/g in group 181 kPa and 708 kPa,respectively. A wide endothelial cell gap with lanthanum nitrate trace leaked out was shown at the group of ultrasound pressure of 181 kPa, and erythrocytes extravasations were found when ultrasound pressure at 708 kPa. Conelusions The pressure amplitude in microbubble enhanced cavitation can effectively regulate and control blood-brain barrier opening in rats.

关 键 词：血脑屏障 微气泡 空化效应 大脑半球 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学] R445.1[医药卫生—临床医学]