凹顶藻萜类化合物协同维生素E辐射防护作用研究  

STUDY ON THE EFFECT OF LAURENCIA EXTRACT COMBINED WITH VITAMIN E FOR PREVENTION OF RADIATION

作　　者：梁惠[1] 刘颖[2] 王青[1] 王文成[1] 马爱国[1] 

机构地区：[1]青岛大学医学院医学营养研究所,青岛266021 [2]青岛大学医学院细胞与分子生物学实验室,青岛266071

出　　处：《营养学报》2015年第4期393-398,共6页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.31171671);国家"十二五"科技支撑计划(No.2012BAD33B01)

摘　　要：目的观察凹顶藻萜类化合物(Laurencia extract,LET)协同维生素E(VE)对亚急性辐射损伤小鼠的防护作用。方法 60只雄性昆明小鼠随机分为正常对照(花生油0.5 ml/kg·d),辐射模型(花生油0.5ml/kg·d),LET低剂量(LET 50 mg+VE 100 mg/kg·d)、LET高剂量(LET 100 mg/kg+VE 100 mg/kg·d)、VE(VE 100 mg/kg·d)、LET(LET 100 mg/kg·d)灌胃,6组,每组10只,对应灌胃,除正常对照组外,其余5组均给予直线加速X射线全身照射,11 d,每次25 CGY,每周5 d,连续4 w。末次灌胃12h后,眼眶取血,行白细胞计数,通过酶联免疫吸附(ELISA)实验测定血清中白介素-2(IL-2)和肿瘤坏死因子-α(TNF-α)水平;处死动物,剥离小鼠股骨行骨髓细胞微核率检测;摘取附睾和睾丸,进行精子畸形率和睾丸精母细胞染色体畸变率检测;摘取胸腺和脾脏,计算脏器指数;采用酶学实验检测肝、肺组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量。结果与正常对照组比较,辐射模型组小鼠白细胞数量明显下降,骨髓细胞微核率、精子畸形率、睾丸染色体畸形率明显升高,脾脏、胸腺指数及血清IL-2水平明显降低,肝、肺组织中SOD活力均明显下降,MDA含量均显著升高,肺组织GSH-Px活力较正常对照组显著下降,差异均具有统计学意义(P<0.05)。一定剂量LET与VE协同作用,可使辐射小鼠白细胞数量显著升高,骨髓细胞微核率、精子畸形率和染色体畸变率显著降低,脾脏及胸腺指数、血清IL-2水平显著提高,肝、肺组织中SOD活力、肺组织GSH-Px活力明显升高,肝、肺组织中MDA含量明显降低,与辐射模型组比较,差异具有统计学意义(P<0.05)。与LET和VE单独用药比较,一定剂量LET与VE协同作用,在提高白细胞数量、血清IL-2水平、免疫脏器指数、肝组织SOD活力、肺组织GSH-Px活力,降低微核率、精子畸形率、染色体畸变率等方面的作用明显增强,经统计学处理,具有显著性�Objective To observe the protective effect of Laurencia extract （LET） ＆amp; vitamin E（VE） in sub-acute radiated mice.Methods Sixty male Kunming mice were randomly assigned to six groups （n=10）： control group, radiation-model group, LET＋ET low-dose group, LET＋ET high-dose group, single-LET group and single-VE group. Except the control group, all mice were treated with X-ray of 25CGY from linear accelerator once a day, five days per week. All mice were sacrdficed in the fourth week. LET Low- and high- dose combination groups were administered by gavaging with the doses of LET （50, 100 mg/kg bw） and VE 100mg/kg bw, respectively. The single-VE group was treated with VE 100 mg/kg bw and the single-LET group was treated with LET 100mg/kg bw. Animals within control group and radiation group were orally given peanut oil 0.5 ml/kg per day. Twelve hours after the last administration, the mice were weighted and sacrificed after taking blood sample from eyeball. Then white blood cell （WBC） count, bone marrow micronucleus rate, chromosome and sperm aberration frequency, spleen and thymus indexes were calculated; Serum IL-12 and TNF-α concentration were measured by enzyme linked immunosorbent assay （ELISA）; The activities of superoxide dismutase （SOD）, glutathione-peroxidase （GSH-Px） and the content of malanydiadehyde （MDA） were examined by biochemical kits. Results Compared with control group, WBC amount, spleen and thymus indexes, serum IL-12 concentration, the activities of SOD in tissues of model groups were obviously decreased, but bone marrow micronucleus rate, chromosome and sperm aberration frequency, the content of MDA were significantly increased （P〈0.05）. Compared with model group, the WBC amount, serum IL-2 concentration, spleen and thymus indexes, activities of SOD, GSH-Px were significantly increased in low- and high-dose LET＋ET groups （P〈0.05）. In addition, the rate of bone marrow micronucleus, chromosome and sperm aberration frequency and the content of

关 键 词：凹顶藻萜类化合物 VE 辐射损伤 免疫调节 抗氧化 

分 类 号：R818[医药卫生—放射医学]