Aspergillus nigerF-01生淀粉糖化酶基因的克隆与序列分析  

Cloning and Sequence Analysis of Gene Encoding Raw-Starch-Digesting-Glucoamylase from Aspergillus niger F-01

作　　者：孙海彦[1] 黎娟华[1] 刘恩世[1] 彭明[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《基因组学与应用生物学》2015年第2期308-312,共5页Genomics and Applied Biology

基　　金：国家自然科学基金(31000029);海南省自然科学基金(ZDFD 20120765);中国热带农业科学院中央级公益性科研院所基本科研业务费专项(ITBB2015RC06);863重点项目(2012AA101204);海南省研究生创新科研课题(Hyb2011-4);海南省引进集成应用专项(YJJC2011004);海南省重大科技项目(ZDZX2013023-1)共同资助

摘　　要：本研究从Aspergillus niger F-01中克隆到了生淀粉糖化酶菌基因的DNA及c DNA序列。序列分析表明,该生淀粉糖化酶基因DNA序列编码区长2 169 bp,c DNA编码区长1 920 bp,该基因含有4个内含子,共编码639个氨基酸,前18个氨基酸为信号肽序列,该氨基酸序列中共含有2个潜在的糖基化位点,软件预测出该酶的分子量约为68.36 k D,等电点为4.2。该研究为今后构建高产的生淀粉糖化酶基因工程菌奠定了基础。DNA and cDNA of raw-starch-digesting-glucoamylase were cloned from Aspergillus niger F-01 in this study. The analysis of sequences revealed that the length of DNA and cDNA of this gene were 2 169 bp and1 920 bp, respectively. The DNA sequence contains 4 introns and encodes a protein of 639 amino acids with a signal peptide of 18 amino acids and two potential glycosylation sites. The calculated molecular weight and isoelectric point of the enzyme were 68.36 kD and 4.2, respectively. The research made a base for construct of high-yield raw-starch-digesting-glucoamylase producing strains.

关 键 词：黑曲霉 生淀粉糖化酶 克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]