IL-35在宫颈癌根治术后复发和转移中的预测价值  

Value of interleukin-35 receptors in predicting recurrence and metastasis of cervical cancer after radical surgery

作　　者：王镇南[1] 黄颢[1] 林琼燕[1] 邹琳[1] 唐志[1] 李淑慧[1] 

机构地区：[1]广东医学院附属医院肿瘤中心,广东湛江524001

出　　处：《广州医科大学学报》2015年第1期54-58,共5页Academic Journal of Guangzhou Medical University

摘　　要：目的:探讨宫颈癌手术前后血清IL-35的差异表达及其表达变化对宫颈癌复发和转移的预测作用。方法:收集2009年1月至2014年1月就诊于本院行宫颈癌手术切除的Ia–IIa期宫颈癌患者73例,同期选择73例本院体检的健康患者(正常对照组),ELISA法检测上述两组以及患者术前、术后血清中IL-35的表达水平,同时根据术后是否复发比较术后复发和稳定组IL-35的含量,分析其与临床预后的关系。结果:与正常对照组比较,IL-35在手术前后患者血清中的表达升高,P<0.05。术后患者血清中的表达(213.79±23.89)pg/m L较术前(398.71±31.09)pg/m L降低,术后复发患者血清IL-35的表达(374.25±27.74)pg/m L较稳定期患者(183.65±34.51)pg/m L明显增高,P<0.05。IL-35高表达的患者的无病生存时间(DFS)为9.115月,总生存时间(OS)为18.031月,较低表达者明显缩短,P均<0.05,Cox分析示IL-35的表达可作为DFS和OS的独立预后因子。结论:手术前后血清IL-35的水平可作为宫颈癌根治术后预测复发和转移的可能生物标志物。Objective： To investigate the predictive effects of different expression levels and the expression changes of pre- and post-operative serum interleukin 35（ IL-35） on recurrence and metastasis of cervical cancer.Methods： The clinical data of 73 patients with Ia-IIa cervical cancer who underwent hysterectomy in our hospital between January 2009 and January 2014 were collected. And a contemporary cohort of 73 healthy patients who underwent health examination in our hospital were included（ normal control group）. The serum IL-35 expression levels were measured in the two groups before and after the operation by enzyme-linked immuno sorbent assay（ ELASA）. Meanwhile,the post-operative content of IL-35 in the recurrence group and stable group were compared according to whether there were recurrence after the operation. And the relationship between the content and the clinical prognosis was analyzed. Results： Compared with the normal control group,the expression level of serum IL-35 increased in the observation group,P〈0. 05. The postoperative expression level of serum IL-35（ 213. 79 ± 23. 89） pg / mL decreased compared with the preoperative expression level（ 398. 71 ± 31. 09）pg / mL; the expression level of serum IL-35 in postoperative patients with recurrence（ 374. 25 ± 27. 74） pg / mL significantly increased compared with that in stable patients（ 183. 65 ± 34. 51） pg / mL,P〈0. 05. The disease free survival（ DFS） and overall survival（ OS） in patients with high expression of IL-35 was 9. 115 months and18. 031 months,respectively,which was significantly shorter than that in patients with lower expression of IL-35,all P〈0. 05. Cox analysis showed that the expression of IL-35 could be independent prognostic factor for DFS and OS. Conclusion： Our results indicate that the expression level of pre- and post-operative serum IL-35 levels may be biomarker in predicting recurrence and metastasis of cervical cancer after curative surgery.

关 键 词：宫颈癌 根治术 IL-35 临床诊断 

分 类 号：R737.33[医药卫生—肿瘤]