荔枝核皂苷对小鼠学习记忆障碍的改善  

Improving effect of Litchi Seed Saponins on learning and memory impairment in mice

作　　者：叶红梅[1] 钟春燕[2] 吕俊华[2] 

机构地区：[1]广州市中医医院药剂科,广东广州510130 [2]暨南大学药学院药理教研室,广东广州510632

出　　处：《广州医科大学学报》2015年第2期10-14,共5页Academic Journal of Guangzhou Medical University

基　　金：广州市中医药中西医结合科研基金资助项目(NO:2009A016)

摘　　要：目的:探讨荔枝核皂苷对D-半乳糖诱致小鼠学习记忆障碍的改善作用。方法:50只昆明种小鼠完全随机分为正常对照组、模型对照组、石杉碱甲组(0.4 mg/kg·d)、荔枝核皂苷低(15 g/kg·d)和高(60 g/kg·d)剂量组。上述动物除空白对照组外,均皮下(sc)注射D-半乳糖(500 mg/kg·d)8周,并于第5周起分别灌胃相应药物。采用水迷宫和自主活动仪测试小鼠学习和记忆能力,测定血清糖基化;醛糖还原酶活性;采用高效液相库仑阵列电化学检测系统检测人神经氨酸酶(NA)、多巴胺(DA)、5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)二羟苯乙酸(DOPAC)的含量变化。结果:荔枝核皂苷明显改善模型小鼠学习和记忆能力,各给药组与模型对照组相比单胺类递质含量差异无统计学意义(P>0.05)。结论:荔枝核皂苷具有改善D-半乳糖诱致小鼠学习记忆障碍的作用;其作用可能与其减少抑制对脑神经细胞的损伤作用有关。Objective： To investigate the improving effect of Litchi Seed extract on learning and memory impairment induced by D-galactose in mice. Methods： Fifty Kunming mice were randomly divided into normal control group,model control group,huperzine A group（ 0. 4 mg / kg · d）,Litchi Seed extract-low dose group（ 15 g/kg·d） and high dose group（ 60 g/kg·d）. Except the normal control group,all groups received Dgalactose（ 500 mg / kg·d） by subcutaneous injection for 8 weeks,and gavages of related drugs at the fifth week,respectively. Water maze test and autonomic activity instrument were used to determine the ability of learning and memory in mice. The serum glycosylation,aldose reductase activities were determined. High performance liquid chromatography-electrochemical detection（ HPLC-ECD） was used to determine the content change of human neuraminidase（ NA）,dopamine（ DA）,5-hydroxytryptamine（ 5-HT）,5-hydroxyindolacetic acid（ 5-HIAA）,dihydroxy-phenyl aceticacid（ DOPAC） were determined. Results： Litchi Seed extract significantly improved the learning and memory ability in model mice. There were no significant differences in the content of monoamine neurotransmitter between medication groups and model control groups（ P〉 0. 05）. Conclusion： Litchi Seed extract can improve the learning and memory impairment induced by D-galactose in mice,which may be related to its reduced inhibition on the damage of brain cells.

关 键 词：荔枝核提取物 D-半乳糖 学习记忆 糖基化产物 单胺类神经递质 小鼠 

分 类 号：R285.5[医药卫生—中药学]