HPLC法测定黄精丸中阿魏酸的含量  

HPLC Determination of Ferulic Acid in Huangjing Pills

作　　者：张颖[1] 王英锋[1] 郝睿[1] 李天一[1] 祝翔[1] 

机构地区：[1]首都师范大学化学系分析测试中心,北京100048

出　　处：《首都师范大学学报（自然科学版）》2015年第4期46-48,共3页Journal of Capital Normal University：Natural Science Edition

摘　　要：目的:建立了HPLC测定黄精丸中阿魏酸含量的方法.方法:用AglientTC-C18(4.6mm×250mm,5μm)色谱柱,0~15min,乙腈-0.085%磷酸溶液(20∶80)为流动相,流速为1mL·min-1,检测波长为316nm,柱温35℃,进样体积10μL,应用安捷伦1200系列液相色谱仪进行测定.结果:阿魏酸与其他成分分离良好,在0.0129μg^0.1161μg范围内呈良好的线性关系(r=0.999998),平均回收率(n=6)为99.96%.结论:该方法简便、准确,重复性好,可用于测定黄精丸中阿魏酸含量.A simple and quick method is described for the determination of Ferulic Acid in Huangjing Pills.Ferulic Acid in the sample was extracted using reversed-phase high performance liquid chromatography（ HPLC）.Chromatography separation was performed using Agilent 1200 series HPLC system with a Aglient TC-C18 column and gradient elution with a mixture of two solvents： solvent A,acetonitrile,solvent B,0.085% phosphoric acid.0 to 15 min A∶ B（ 20∶ 80）.The effluent was monitored using a VWD detector set at 316nm.The flow rate was set at 1mL·min- 1 and the injection volume was 10 μL.The column temperature was maintained at 35℃.Ferulic Acid was well separated and the linearity was obtained with in the range of 0.012 9μg ~ 0.116 1μg（ r = 0.999 998）; The average recovery（ n = 6） was 99.96%.The method is simple,accurate,reproducible,and can be used for determination of Ferulic Acid in Huangjing Pills.

关 键 词：高效液相色谱 黄精丸 阿魏酸 

分 类 号：R284.1[医药卫生—中药学]