醛酮还原酶家族1的C1亚型在胃癌的表达及其作用探讨  

Preliminary Discussion on the Expression and the Effect of Aldo-keto Reductase Family 1 Member C1 in Gastric Carcinoma

作　　者：刘畅[1] 陆慧琪[1] 陈佳[2] 祝丽双[1] 李倩[3] 韩焕兴[1] 

机构地区：[1]第二军医大学附属长征医院,上海200433 [2]第二军医大学附属东方肝胆外科医院,上海200433 [3]郑州大学第二附属医院,河南郑州450000

出　　处：《现代生物医学进展》2015年第19期3621-3623,3656,共4页Progress in Modern Biomedicine

基　　金：上海市科学技术委员会项目(2014DFA33010)

摘　　要：目的:研究AKR1C1(Aldo-keto reductase family 1 member C1)在胃癌中的表达,并初步探讨其在胃癌发生和发展中的作用。方法:采用胃癌组织芯片和免疫组化分析方法,检测60例胃癌患者癌组织及癌旁正常组织AKR1C1的表达;实时荧光定量PCR和蛋白免疫印迹western blot检测胃癌细胞株SGC-7901 AKR1C1的表达;在胃癌细胞株SGC-7901中转染沉默AKR1C1的sh RNA质粒及空质粒,MTT比色法检测各实验组细胞增殖。结果:组织芯片和免疫组化结果显示,与正常组织相比,胃癌组织中AKR1C1呈高表达;实时荧光定量PCR和western blot观察可以发现胃癌细胞株SGC-7901高表达AKR1C1;MTT比色法检测发现,转染沉默AKR1C1的sh RNA质粒组与空质粒对照组相比,SGC-7901细胞的增殖受到明显抑制,差异有统计学意义(P<0.05)。结论:AKR1C1与癌细胞的增殖有关,可能是其参与或间接参与了癌细胞的生长周期,为胃癌的发生及细胞增值提供了新的研究思路和方向。Objective： To investigate the expression ofAKR1C1 （aldo-keto reductase family 1 member C1） in gastric carcinoma and discuss its role in gastric carcinogenesis and development. Methods： Gastric cancer tissue microarray and immunohistochemical method were used to detect the expression of AKR1C1 in gastric carcinoma tissue rnicroarray of 60 patients. Use the method of real-time fluorescence quantitative PCR and protein western blot to detect the expression of AKR1C1 in gastric cancer cell lines SGC-7901. Transfect gene silencing AKR1C1 short hairpin RNA plasmid and empty plasmid in gastric cancer cell lines SGC-7901 and use the method of MTT colorimetric assay to detect cell proliferation. Results： Tissue rnicroarray and immunohistochemical method showed that the expression of AKR1C1 in the cancer tissue was higher than that in normal tissue. High expression of AKR1C1 in gastric cancer cell lines SGC-7901 was detected by the method of real-time fluorescence quantitative PCR and protein western blot. The method of MTT colorimetric assay found that cell proliferation was significantly inhibited in gene silencing AKR1C1 short hairpin RNA plasmid transfection group, compared with empty plasmid and blank group. And the difference has statistical significance （P 〈 0.05）.Conclusions： AKR1C1 has relationship with proliferation of cancer cell. The reason may be that AKR1C1 is directly or indirectly involved in the growth cycle of cancer cell.Provide new research ideas and directions for gastric cancer proliferation occurs.

关 键 词：AKR1C1 SGC-7901细胞 增殖 

分 类 号：R735.2[医药卫生—肿瘤]