电针预处理对脑缺血再灌注后小胶质细胞活化状态的影响  

Effect of Electroacupuncture Pretreatment on the State of Microglial Activation after Cerebral Ischemia and Reperfusion in Rat

作　　者：杨蕾[1] 翟茜[1] 江涛[1] 邓斌[1] 王强[1] 

机构地区：[1]第四军医大学西京医院麻醉科,陕西西安710032

出　　处：《现代生物医学进展》2015年第20期3826-3829,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81173394)

摘　　要：目的:观察电针预处理对脑缺血再灌注后小胶质细胞活化状态的影响。方法:成年雄性SD大鼠随机分为假手术组(sham)、缺血组(MCAO)、电针处理组(EA+MCAO)三组。采用大脑中动脉栓塞(MCAO)诱导大鼠局灶性脑缺血再灌注模型。缺血再灌注后6 h、24 h、3 d和7 d取材,运用Western blot及免疫荧光技术检测缺血半暗带小胶质细胞活化状态以及M1/M2型特异性标志分子的表达水平。结果:脑缺血再灌注后,小胶质细胞被激活,数量表达增加(P<0.05,vs.sham组),形态从静息状态的分支状转变为圆形的阿米巴状。M1型标志分子i NOS主要表达于缺血再灌注后24小时(P<0.05,vs.sham组),M2型标志分子Arginase主要表达于缺血再灌注后7天(P<0.05,vs.sham组)。电针预处理上调Arginase的表达水平,下调i NOS的表达水平(P<0.05,vs.MCAO组)。结论:缺血再灌注后小胶质细胞被激活,电针预处理促使活化的小胶质细胞由M1向M2转化。Objective： To observe the effect of electroacupuncture （EA） pretreatment on the state of microglial activation after cerebral ischemia and reperfusion in rat. Methods： Adult male SD rats were divided into 3 groups： sham, MCAO, and EA＋MCAO. Cerebral ischemic injury was induced by transient middle cerebral artery occlusion （MCAO）. The morphology of microglia was demonstrated by immunofluorescent confocal images. The expression of M1/M2 phenotypes of microglia in the cerebral ischemic penumbra was analyzed by Western blot. Results： After reperfusion, the microglia was activated. The expression of microglial marker Iba-1 in FR group was increased （P〈0.05,vs.sham）. The morphology of microglia shifted from the ramified to the rounded amoeboid-like appearance. Quantitative analysis showed the peak expression of M1 phenotype marker iNOS occurred at 24 hour after reperfusion （P〈0.05,vs.sham）, while the marker of M2 microglia Arginase maximized at 7 days after reperfusion （P〈0.05,vs.sham）. Electroacupuncture pretreatment increased the expression of Arginase and decreased the expression of iNOS （P〈0.05,vs.MCAO）. Conclusions： After ischemia and reperfusion, the microglia was activated, Electroacupuncture pretreatment made the activated microglia shift from M1 to M2 phenotype.

关 键 词：电针 缺血再灌注损伤 小胶质细胞 M1型 M2型 

分 类 号：Q95-3[生物学—动物学] R743[医药卫生—神经病学与精神病学]