GCC mRNA和hTERT mRNA在腹腔镜结直肠癌根治术患者外周血中的表达及意义  

Expressions and Significance of GCC mRNA and hTERT mRNA in the Peripheral Blood of Patients with Laparoscopic Colorectal Resection

作　　者：张炎[1] 刘刚[1] 黄云[1] 邹贵军[1] 王育红[1] 

机构地区：[1]中国人民解放军海军总医院,北京100048

出　　处：《现代生物医学进展》2015年第20期3882-3885,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年科学基金项目(30901795)

摘　　要：目的:探讨腹腔镜和开腹手术对结直肠癌根治术患者外周血鸟苷酸环化酶C(GCC)m RNA和人端粒酶逆转录酶(h TERT)m RNA表达的影响及其意义。方法:按照随机数字表法将2009年8月-2014年4月我院收治的结直肠癌患者分为腹腔镜组和开腹手术组,通过逆转录-聚合酶链反应技术(RT-PCR)检测并比较两组患者外周血GCC m RNA和h TERT m RNA的表达情况。结果:与开腹手术组相比较,腹腔镜组患者手术时间长、术中出血量少、术后排气早、住院时间短,差异有统计学意义(P<0.01);两组患者手术前GCC m RNA和h TERT m RNA阳性率无显著差异(P>0.05);两组患者手术后GCC m RNA和h TERT m RNA阳性率显著升高,差异有统计学意义(P<0.05)。结论:腹腔镜和开腹手术均会增加结直肠癌患者血行微转移,但腹腔镜结直肠根治术不会增加术后肿瘤血循环微转移的危险性。Objective： To investigate the expressions and significance ofGCC mRNA and hTERT mRNA in the peripheral blood of patients with colorectal cancer. Methods： Patients with colorectal cancer in our hospital from August 2009 to April 2014 were divided into the laparoscopic group and the open surgery group. Then the RT-PCR was used to detect the expressions of GCC mRNA and hTERT mRNA of patients, and the results were compared between the two groups. Results： The operation time of laparoscopic group was longer than that of the open surgery group, but the blood loss, the postoperative exhaustation and the hospitalization were less than those of the open surgery group, and the differences were statistically significant （P〈0.05）. There was no statistically significant difference about the positive expression rate of GCC mRNA and hTERT mRNA in two groups before the operation （P〉0.05）. The positive rates of GCC mRNA and hTERT mRNA after operation in two groups were higher than before, and the differences were statistically significant （P〈0.05）. Conclusion： Using laparoscopic and open radical operation treatment for colorectal cancer will increase the likelihood of blood micrometastasis, but laparoscopic colorectal resection dose not increase the risk of postoperative micrometastasis of tumor blood circulation.

关 键 词：腹腔镜 开腹手术 结直肠癌根治术 鸟苷酸环化酶C 端粒酶逆转录酶 

分 类 号：R735.3[医药卫生—肿瘤]