miR-181b-5p对于卡波西肉瘤细胞SLK细胞增殖和凋亡的影响  

The Effects of MicroRNA-181b-5p on Proliferation and Apoptosis of Human Kaposi's Sarcoma Cell Line SLK

作　　者：丁媛[1,2] 吴秀娟[2] 向芳[2] 康晓静[2] 于世荣[2] 冯燕艳[2] 王红娟[2] 普雄明[2] 

机构地区：[1]新疆医科大学,新疆乌鲁木齐830054 [2]新疆维吾尔自治区人民医院皮肤性病科,新疆乌鲁木齐830001

出　　处：《现代生物医学进展》2015年第21期4005-4008,4013,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金地区科学基金项目(81260311);新疆维吾尔自治区自然科学基金项目(2014211A059)

摘　　要：目的:通过脂质转染mi R-181b-5p模拟物及抑制物到卡波西肉瘤细胞系SLK,首次研究mi R-181b-5p对卡波西肉瘤细胞SLK细胞增殖、凋亡生物学功能的影响,为进一步研究mi R-181b-5p在卡波西肉瘤发病机制中的作用奠定理论基础。方法:应用脂质体对人卡波西肉瘤细胞株SLK进行转染,对转染mi R-181b-5p模拟物及抑制物后的卡波西肉瘤细胞株行MTT检测观察细胞增殖曲线;应用流式细胞仪行检测转染48小时后各组细胞凋亡率比较。结果:与阴性对照组比较,mi R-181b-5p模拟物组细胞的增殖能力明显上升,(P<0.01)。mi R-181b-5p抑制物组细胞的增殖能力明显下降,(P<0.01)。mi R-181b-5p抑制物阴性对照组细胞的增殖能力无明显变化。流式细胞仪测定SLK细胞转染mi R-181b-5p模拟物后凋亡率(5.5±0.6)%,与阴性对照组凋亡率(7.6±0.4)%相比,差异有统计学意义,明显下降(P<0.05);mi R-181b-5p抑制物组凋亡率(14.8±1.0)%明显增高,有统计学差异(P<0.05)。结论:通过转染mi R-181b-5p模拟物/抑制物,发现其对SLK细胞的增殖、凋亡造成一定影响,进一步开展mi R-181b-5p生物学特性和其相关靶基因验证的相关研究有十分重要的意义。Objective： miR-181b-5p mimics and inhibitors were transfected into Kaposi＇s sarcoma cell line SLK through liposomal transfection. The effect of miR-181b-5p on SLK cell proliferation and apoptosis of biological activities were studied to provide theoretical basis for further investigation on the role of miR-181b-5p in the pathogenesis of Kaposi＇s sarcoma. Methods： Human Kaposi＇s sarcoma cell line SLK was transfected by liposomes. The proliferation of SLK cell was assessed by MTT assay; apoptosis rates of each group were determined by flow cytometry at 48 hours after transfection. Results： Compared to the negative control group, proliferative capacity of miR- 181 b-5p mimics has increased significantly after transfection （P 〈 0.01）, proliferative capacity of miR- 181 b-5p inhibitor has de- creased significantly after transfection （P 〈 0.01）. Flow cytometry showed that, apoptosis rate of the SLK cells has decreased significantly after transfection with miR-181b-5p mimics （5.5± 0.6） % compared to that of corresponding negative control （7.6± 0.4） % （P〈0.01）. Apoptosis rate of the SLK ceils has increasedsignificantly after transfection with miR-181b-5p inhibitor （14.8± 1.0）compared to that of corresponding negative control（7.8 ± 0.5） % （P 〈 0.01）. Conclusions： Transfection with miR- 181 b-5p mimics/inhibitors significantly af- fected SLK cell proliferation and apoptosis. Further researches on biological characteristics of miR-181 b-5p and validation of the associ- ated target genes are therefore of great significance.

关 键 词：miR-181b-5p 卡波氏肉瘤 增殖 凋亡 

分 类 号：R739.5[医药卫生—肿瘤]