骨巨细胞瘤组织S100A8和S100A9表达及与肿瘤影像学表现的相关性分析  

Expression of S100A8 and A100A9 in giant cell tumor of bone and its relation with CT and MR imaging findings

作　　者：廖金生[1,2] 丁晓毅[2] 许顺良[1] 

机构地区：[1]浙江大学医学院附属第一医院放射科,浙江杭州310003 [2]上海交通大学医学院附属瑞金医院放射科,上海200025

出　　处：《浙江大学学报（医学版）》2015年第3期329-334,共6页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(81072188)

摘　　要：目的：检测骨巨细胞瘤患者肿瘤组织中S100A8、S100A9mRNA及蛋白的表达水平，探讨其与骨巨细胞瘤的影像学及生物学行为之间的关系。方法：收集2009年1月至2012年6月在上海交通大学医学院附属瑞金医院诊治的43例骨巨细胞瘤患者的临床资料，并采集骨巨细胞瘤新鲜组织和6例正常成人股骨的骨髓组织，运用半定量逆转录PCR及蛋白质印迹法检测肿瘤组织中S100 A8、S100 A9 mRNA及其蛋白表达水平，并分析与不同肿瘤影像学表现如CT检查显示的骨质破坏程度，MRI检查显示的实性成分比例及周边水肿程度等关系。结果：43份骨巨细胞瘤组织中有40份表达S100 A8、S100 A9 mRNA及其蛋白，与正常骨髓组织比较，骨巨细胞瘤组织S100 A8、S100 A9 mRNA及其蛋白表达增加，差异均有统计学意义（均P＜0．05）。影像学不同实质成分比例的骨巨细胞瘤患者S100 A8蛋白表达差异具有统计学意义（ P ＜0．05）；不同骨质破坏程度的骨巨细胞瘤患者S100 A9蛋白表达差异也有统计学意义（ P ＜0．05）。结论：骨巨细胞瘤组织中S100 A8和S100 A9表达增加，并与骨巨细胞瘤骨质破坏程度及实性成分比例、水肿程度具有一定相关性，S100 A8、S100 A9可能参与骨巨细胞瘤的发生发展。Objective： To investigate the mRNA and protein expression levels of S100A8 and S100A9 in giant cell tumor （ GCT ） of bone, and its relation with radiological findings and biological behavior .Methods：Forty three patient with GCT of bone admitted in Ruijin Hospital Shanghai Jiaotong University School of Medicine from January 2009 to June 2012 were enrolled in the study .The expression levels of S 100 A8 and S100 A9 mRNA and protein were detected by using semiquantitative RT-PCR and Western blotting in 43 specimens of GCT and 6 specimens of normal bone marrow .The CT and MRI findings of patients were retrospectively reviewed , its relation with tissue expression of S100 A8 and S100 A9 was analyzed .Results： Among 43 GCT cases 40 showed positive expression of S 100 A8 and S100 A9 mRNA and protein , and the expression levels were significantly higher than those in normal bone marrow （P〈0.05）.The expression level of S100A8 protein was significantly different in bone GCT with different composition ratio on MRI （ P 〈0 .05 ） .The expression level of S100 A9 protein was significantly different in GCT with different degree of bone destruction on CT scan （ P 〈0.05 ）.Conclusion： The expression of S100A8 and S100A9 mRNA and protein is up-regulated in GCT of bone .The expression of S100A8 and S100 A9 is associated with the real composition ratio and the degree of bone destruction, respectively, indicating that S100A8 and S100A9 may be involved in the biological behavior of bone GCT .

关 键 词：巨细胞瘤 骨/病理学 巨细胞瘤 骨/放射摄影术 RNA 信使/遗传学 S100蛋白质类/生物合成 体层摄影术 X线计算机 磁共振成像 

分 类 号：R738.1[医药卫生—肿瘤]