bFGF和PDGF-B蛋白在脑缺血再灌注大鼠模型中的表达和血管形成的研究  

The expression of basic fibroblast growth factor,platelet-derived growth factor B chain and its effects on angiogenesis in rats following cerebral ischemia/reperfusion

作　　者：刘伯芹 王国峰 仲玲 赵仁亮[2] 

机构地区：[1]青岛市第九人民医院神经内科,266002 [2]青岛大学附属医院神经内科

出　　处：《卒中与神经疾病》2015年第4期198-202,共5页Stroke and Nervous Diseases

摘　　要：目的探讨脑缺血再灌注大鼠不同时间碱性成纤维细胞生长因子(bFGF)和血小板源性生长因子-B(PDGF-B)的表达水平及其与血管形成的关系。方法采用线栓法制备大鼠大脑中动脉局灶性脑缺血再灌注(MCAO/R)模型,分为假手术组和MCAO/R组,MCAO/R组缺血2 h后根据再灌注时间窗的不同分为0、6、24 h、3、7、14、21 d共7组,应用HE染色观察病理变化并测定脑梗死体积,用免疫组化法检测CD34蛋白的表达水平并计数微血管密度(MVD),用免疫组化法检测bFGF和PDGF-B蛋白的表达水平。结果 bFGF蛋白表达水平在MCAO/R6 h后即开始升高,3 d到达高峰,7 d开始降低。PDGF-B蛋白表达水平在MCAO/R24 h后明显升高,3 d到达高峰,7 d有所下降,持续到14 d左右。MVD表达在MCAO/R3 d开始升高,7 d到达高峰。bFGF和PDGF-B蛋白表达水平与MVD变化呈正相关(P<0.01)。结论局灶性脑缺血再灌注损伤可诱导bFGF、PDGF-B和新生血管表达增加,激活内源性脑保护机制。Objective To investigate the effects of angiogenesis and the expression changes of basic fibroblast growth factor （bFGF） and platelet-derived growth factor B chain（PDGF-B ） in rats following focal cerebral ischemia/reperfusion. Methods A model of the middle cerebral artery occlusion 2h / reperfusion （MCAO/R） in rats was performed with the intraluminal filament occlusion. Rats were divided into sham oper- ation group and MCAO/R group. Two hours after ischemia the MCAO/R group was divided into 7 subgroups according to reperfusion time difference, including 0, 6, 24 h, 3, 7, 14, 21 d. Brain sections were stained with hematoxylin and eosin （HE） for surveying the volume of infraction. The expression of microvascular den- sity（MVD）was determined by immunohistochemical staining. The expression of bFGF and PDGF-B protein were determined by mmunohistochemical staining. Results The expressions of bFGF began to increase at 6 h after ischemia/reperfusiora It reached climax at 3 d and reduced after 7 d. The expressions of PDGF-B were significantly increased at 24 h after ischemia/reperfusion, with the first peak at 3 d and the second peak was at 14 d. The expressions of MVD began to increase at 3 d after ischemia/reperfusion and continue to 14 d. There was a significant positive correlation be^ween expression of bFGF, PDGF-B and change of MVD（P^0. 05）. Conclusions Ischemia and reperfusion injury can induce expression of bFGF, PDGF-B and angiogenesis, which might play an important role in the internal protection of neurons.

关 键 词：碱性成纤维细胞生长因子 血小板源性生长因子-B 血管形成 脑缺血 

分 类 号：R743[医药卫生—神经病学与精神病学]