蛋白激酶D1在大鼠骨髓源性内皮祖细胞中的促血管新生作用  

Angiogenesis of protein kinase D1 in bone marrow-derived endothelial progenitor cells of rats

作　　者：刘暖[1] 杨雷[1] 毛秉豫[1] 徐国昌[1] 叶松山[1] 张培华[1] 张瓅芳 

机构地区：[1]南阳理工学院医学实验中心,河南南阳473004

出　　处：《中国药理学通报》2015年第9期1259-1264,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81473438;81202791;81173372);河南省中医内科学重点学科支撑课题(豫教高[2012]186号)

摘　　要：目的探讨蛋白激酶D1(PKD1)对大鼠骨髓源性内皮祖细胞(EPCs)黏附、迁移、增殖、血管形成能力及内皮型一氧化氮合成酶(e NOS)表达的影响。方法体外培养、分离和鉴定大鼠骨髓源性EPCs,观察PKD1及其特异性阻断剂CID755673对EPCs的黏附、迁移、增殖、血管形成能力的影响,以及对EPCs中e NOS的mRNA表达和蛋白表达的影响。结果 EPCs体外细胞培养实验表明,PKD1可明显促进EPCs的黏附、迁移和增殖,提升EPCs的血管形成能力,上调EPCs中e NOS的mRNA表达和蛋白表达水平。结论 PKD1具有调控EPCs促血管新生的作用,其促血管新生的作用可能以一种依赖e NOS的方式进行。Aim To explore the effect of protein kinase D1（ PKD1） on adherence,migration,proliferation,microvascular formation,and e NOS expression in bone marrow-derived endothelial progenitor cells（ EPCs） of rats. Method The EPCs were isolated from bone marrow of rats,cultured and detected,the effects of PKD1 and its specific blocking agent CID755673 on adhesion, migration, proliferation, or microvascular formation were observed,as well as mRNA and protein expression of endothelial nitric oxide synthase（ e NOS）in EPCs. Results PKD1 significantly promoted adhesion,migration,proliferation and microvascular formation of EPCs,and upregulated the mRNA and protein expression of e NOS in EPCs,according to the cell culture experiments of EPCs in vitro. Conclusion PKD1has the role of angiogenesis through regulation of EPCs,which might be dependent on e NOS.

关 键 词：蛋白激酶D1 内皮祖细胞 血管新生 内皮型一氧化氮合成酶 迁移 增殖 

分 类 号：R-332[医药卫生] R329.24