何首乌对泼尼松致大鼠股骨微结构及生物力学改变的预防作用  

Preventive effect of Polygonum Multiflorum on deteriorated micro-structure and biomechanical properties induced by prednisone

作　　者：周满如[1] 李近[1] 吴敬开 曾小斌[2] 陈景锋[1] 崔燎[1,2] 刘钰瑜[1] 

机构地区：[1]广东医学院药理学教研室,广东湛江524023 [2]广东医学院广东天然药物研究与开发重点实验室,广东湛江524023

出　　处：《中国药理学通报》2015年第9期1273-1279,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81102450);广东省科技计划资助项目(No 2012B031800225);广东省教育厅特色创新项目(自然科学类;No 2014KTSCX084)

摘　　要：目的探讨何首乌对泼尼松致大鼠股骨微结构及生物力学改变的预防作用。方法 6月龄SD♂大鼠按体重随机分成9组:空白对照组、泼尼松组、罗盖全组、何首乌30%醇提取物(高、中、低剂量)组、何首乌总提取物(高、中、低剂量)组。采用泼尼松连续灌胃21周建立骨丢失动物模型,药物预防组在给予泼尼松同时灌胃给予不同的药物。实验结束后,取右股骨进行Micro-CT扫描及三维重建,观察松质骨骨微结构及检测相关参数,并进行生物力学测定。结果与空白对照组比较,模型组出现骨微结构的破坏,BV/TV下降28.6%(P<0.05),骨生物力学性能降低,刚度下降29.7%(P<0.01)。与模型组比较。Aim To investigate the preventive effect of Polygonum Multiflorum（ PM） on the deteriorated micro-structure and biomechanical properties induced by prednisone. Methods Ninety 6- month-old male Sprague-Dawley rats were randomly divided into nine groups,which were control,prednisone,CAL,30%ethanol eluent of the PM（ H,M,L）,PM（ H,M,L）.Prednisone was gavaged to rat for 21 weeks as model group of osteoporosis. Meanwhile,tested herbal abstract were orally administrated to the modeled rats induced by prednisone. At the end of the experiment,the right femur was collected for micro-CT scanning,three-dimensional reconstruction and biomechanical test. Results Compared with the control group,model group showed destruction of bone microarchitecture,BV / TV fell 28. 6%（ P〈0. 05）,bone biomechanical parameters decreases,and stiffness fell 29. 7%（ P〈0. 01）. Compared with the model group, positive group had significantly improved effect on bone microarchitecture,and biomechanical parameters,BV / TV increased 46. 7%（ P〈0. 01）,and stiffness increased25. 9%（ P〈0. 01）. 30% ethanol eluent of the PM（ M,L） dose may improve bone microstructure by increasing BV / TV 46. 7%（ P〈0. 01）,40. 0%（ P〈0. 05） respectively,PM（ H） may improve the biomechanical parameters by increasing stiffness 24. 7%（ P〈0. 05）,and 30% ethanol eluent of the PM（ H） and PM high-dose may improve the biomechanical parameters,but not as positive group. Conclusions Prednisone reduces biomechanical properties of rat femur and deteriorates femoral microstructure. 30% ethanol eluent of the PM（ M,L） and PM（ H） plays a preventive role in the changes of micro-structural and biomechanical properties by prednisone,and increases BMD,whereas other groups have no significant preventive effect.

关 键 词：何首乌 泼尼松 骨微结构 骨生物力学 骨质疏松 大鼠 

分 类 号：R-332[医药卫生] R282.71