拟黑多刺蚁醇提物抗抑郁作用研究  

Antidepressive-like effect of alcoholic extract of Polyrhachis vicina Roger

作　　者：韦桂宁[1] 楚世峰[2] 苏华[1] 何飞[1] 苏启表[3] 卢文杰[1] 陆国寿[1] 黄周锋[1] 谭晓[1] 林霄[1] 曾宪彪[1] 韦宝伟[1] 陈乃宏[4] 

机构地区：[1]广西中医药研究院药理所,广西南宁530022 [2]湖南中医药大学中医学院,湖南长沙410208 [3]广东药学院健康学院,广东广州510006 [4]湖南中医药大学药学院,湖南长沙410208

出　　处：《中国药理学通报》2015年第9期1280-1286,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81360653;81560663);广西自然科学基金资助项目(No 2011GXNSFA018258);广西中医药民族医药继承创新工程课题(No GZPT13-29)

摘　　要：目的观察拟黑多刺蚁醇提物抗抑郁作用,并对其作用机制进行初步分析。方法拟黑多刺蚁醇提物通过乙醇提取得到。采用悬尾试验、强迫游泳试验等行为绝望模型和利血平诱导的抑郁模型观察拟黑多刺蚁醇提物抗抑郁作用。结果拟黑多刺蚁醇提物8、4 g·kg-1剂量组均能明显缩短小鼠在TST和FST的悬尾不动时间和游泳不动时间(P<0.05);拟黑多刺蚁醇提物8 g·kg-1剂量组可提高抑郁症大鼠体温和糖水摄取量(P<0.05);拟黑多刺蚁醇提物8、2 g·kg-1剂量组可拮抗利血平大鼠眼睑下垂,减少运动不能时间(P<0.05,P<0.01);拟黑多刺蚁醇提物8 g·kg-1可明显提高抑郁症大鼠血清、海马组织、大脑皮层的5-羟色胺、去甲肾上腺素水平及超氧化物歧化酶的活性(P<0.05);拟黑多刺蚁醇提物对所述正常组各指标无影响。结论拟黑多刺蚁醇提物具有明显的抗抑郁作用,可能通过对神经递质代谢及对神经细胞抗氧化作用的调节来实现。Aim To investigate the antidepressive-like effect of ethyl alcohol extract of Polyrhachis vicina Roger（ EAPR）,and its mechanism. Methods EAPR wasprepared by ethanol extraction. Its anti-depressive effect was investigated by tail suspension test（ TST） and forced swimming test（ FST）. Furthermore,repeateddoses of reserpine was used for preparing the depressive rats. Results EAPR has definitely anti-depressive effect,as evidenced by the decreased immobility time in FST and TST at the doses of 8 and 4 g·kg- 1（ P〈0. 05）. In the repeated reserpine evoked depressive rats,EAPR antagonized the symptoms induced by monoamines depletion and attenuated the anhedonia,as manifested by reversed hypothermia,akinesia and sucrose consumption at the doses of 8 and 2 g · kg- 1（ P〈0. 05,P〈0. 01）. Neuro-chemical studies showed that AFPR significantly increased the concentration of monoamines, including 5-hydroxytryptamine（ 5-HT）and noradrenaline（ NA） at the dose of 8 g·kg- 1（ P〈0. 05）,and had no effect on normal rats. Furthermore,EAPR increased the activity of superoxide dismutase（ SOD） in serum,hippocampus and cerebral cortex at the dose of 8 g·kg- 1（ P〈0. 05）. Conclusion EAPR possesses the definite antidep ressive properties,connected with the regulation of neurotransmitter metabolism and the nerve cells antioxidant effect.

关 键 词：拟黑多刺蚁 抑郁 作用机制 5-羟色胺 去甲肾上腺素 超氧化物歧化酶 

分 类 号：R-332[医药卫生] R284.1