血管紧张素(1-7)、Mas受体在大鼠肝纤维化形成过程中的作用  

Role of angiotensin(1-7) and Mas receptor in rat liver fibrosis progression

作　　者：武淑君[1,2] 霍丽娟[1] 王晋江[1] 张婕[1] 贾慧[1] 

机构地区：[1]山西医科大学第一医院消化科,太原030001 [2]山西医科大学

出　　处：《中华消化病与影像杂志（电子版）》2015年第4期17-21,共5页Chinese Journal of Digestion and Medical Imageology(Electronic Edition)

基　　金：山西省卫生厅科研课题计划(201201064)

摘　　要：目的探讨血管紧张素(1-7)[Ang(1-7)]、Mas受体在大鼠肝纤维化形成过程中的作用。方法成年雄性Wistar大鼠36只,随机选取6只作为正常对照组,余30只制备四氯化碳诱导的大鼠肝纤维化模型。建模15、30、45、60、75 d分别随机处死6只大鼠,HE及Masson染色观察各组大鼠肝组织的病理变化,ELISA法检测肝组织Ang(1-7)的表达水平,免疫组织化学法观察肝组织Mas受体蛋白、Ⅰ型胶原(ColⅠ)的定位及表达,实时PCR及Western印迹法分别检测肝组织Mas受体mRNA及其蛋白的表达。结果随着建模时间的延长,大鼠肝纤维化的进展,大鼠肝组织Ang(1-7)、Mas受体mRNA及其蛋白表达水平呈逐渐增高的趋势,且组间差异均有统计学意义(除外建模60 d与建模75 d比较,F值分别为49.05、169.65、45.76、77.31,均P<0.01);而ColⅠ的表达水平逐渐增高,且组间差异均有统计学意义(F=51.02,P<0.01)。结论 ColⅠ与大鼠肝纤维化程度密切相关,Ang(1-7)、Mas受体在肝纤维化形成过程中发挥了重要作用。Objective To explore the role of angiotensin （1-7） LAng （1-7） J and Mas receptor in rat liver fibrosis progression. Methods From 36 adult male Wistar rats, 6 were randomly selected as the normal control group, and the remaining 30 were given subcutaneous injection of 40% carbon tetrachloride （ CC14 ） to induce liver fibrosis. Six were killed, respectively, in the modeling days of 15th, 30th, 45th, 60th and 75th. Histopathological study of liver tissue was done with hematoxylin-eosin （HE） and rapid Masson staining. Enzyme-linked immunosorbent assay （ELISA） was used to determine the levels of Ang （ 1-7 ） of liver homogenates. The position and expression of Mas receptor protein and collagen Ⅰ （Col Ⅰ ） were observed by immunohistochemistry. Real-time PCR and Western blotting were respectively used to detect the expression of Mas receptor mRNA and protein. Results With the extended modeling time and the rat liver fibrosis progression, the levels of Ang（ 1-7 ） and Mas receptor mRNA and protein showed an upward trend, and the differences between groups were statistically significant （ except 60 d vs 75 d, F value was 49.05, 169.65, 45.76, 77.31 respectively, all P 〈 0.01 ）. The expression of Col Ⅰ gradually increased in liver tissue, and the difference was statistically significant （ F = 51. 02, P 〈 0.01 ）. Conclusion Col Ⅰ is closely related to liver fibrosis, Ang （ 1-7 ） and Mas receptor play an important role in the development of liver fibrosis.

关 键 词：肝硬化 血管紧张素类 MAS受体 Ⅰ型胶原 

分 类 号：R575.2[医药卫生—消化系统]