125I粒子和60Co γ射线照射对A549及BEAS-2B细胞生物学效应的影响  

The biological effects of 125I seeds and 60Co γ-rays on A549 and BEAS-2B cells

作　　者：赵真真[1] 茅爱武[3] 王忠敏[2] 刘芬菊[4] 曹燕[3] 贾一平[3] 

机构地区：[1]上海交通大学医学院附属仁济医院影像科,200001 [2]东南大学医学院、上海交通大学医学院附属瑞金医院卢湾分院 [3]上海交通大学医学院附属同仁医院介入科 [4]苏州大学医学部放射医学与防护学院

出　　处：《中华放射医学与防护杂志》2015年第8期575-579,共5页Chinese Journal of Radiological Medicine and Protection

基　　金：上海市卫计委科研项目（20114014）;中国博士后科学基金（2015T80489）

摘　　要：目的 探讨125I粒子和60Co γ射线对非小细胞肺癌（NSCLC） A549细胞和正常支气管上皮BEAS-2B细胞生物学效应的影响.方法 A549、BEAS-2B细胞均行125I粒子和60Coγ射线不同剂量照射;集落形成实验检测细胞存活分数;流式细胞术检测细胞周期和细胞凋亡率;Western blot检测凋亡相关蛋白的表达水平.结果 A549细胞在4、6、8 Gy照射时,125I粒子组细胞克隆存活分数较60Co组降低更明显（t=6.06、9.42、4.90,P＜0.05）.A549细胞在4 Gy时,G1期细胞比例125I粒子组为70.67％±1.49％,60Co组为59.59％ ±0.71％（t=10.77,P＜0.05）;细胞凋亡率125I粒子组为18.09％±0.73％,60Co组为9.81％±0.16％（t=19.40,P＜0.05）.125I粒子照射明显上调Bax、cleaved Caspase-3蛋白的表达,同时下调Bcl-2蛋白的表达.但不同射线同一剂量或相同射线不同剂量下,BEAS-2B细胞的凋亡率及凋亡相关蛋白的表达无明显变化.结论 125I粒子持续低剂量率照射较60Co γ射线高剂量率照射抑制A549细胞增殖的效应更明显.Bcl-2/Bax蛋白比失衡,最终致Caspase-3蛋白的活化在125I粒子持续低剂量率照射抑制肿瘤细胞增殖的效应中可能发挥重要的作用.Objective To investigate the biological effects of 125I seeds and 60Co γ-rays on the non-small cell lung cancer cells A549 and the normal bronchial epithelium cells BEAS-2B.Methods A549 and BEAS-2B cells were irradiated with 125I seeds and 60Co γ-rays.The survival fraction was detected by colony formation assay.The cell cycle and cell apoptotic ratio were detected by flow cytometry.The expression of cell apoptotic related proteins was examined by western blot.Results After irradiation with different doses,the survival of A549 cells irradiated with 125I seeds was lower than that irradiated with γ-rays （t =6.06,9.42,4.90,P 〈0.05）.After irradiation with 4 Gy of 125I seeds and 60Co γ-rays,the G1 phase percentages of A549 cells were 70.67% ± 1.49% and 59.59% ± 0.71% （t =10.77,P 〈 0.05）,and the apoptotic ratios of A549 cells were 18.09% ±0.73% and 9.81% ±0.16% （t =19.40,P〈 0.05）,respectively.125I seeds irradiation remarkably up-regulated the expressions of Bax and cleaved Caspase-3 proteins,down-regulated the expression of Bcl-2 proteins compared with 60Co γ-rays irradiation on A549 cells.However,the apoptotic ratio and the expressions of apoptosis-related proteins in BEAS-2B cells had little difference between two types of radiation.Conclusions The anti-proliferative effect of 125I seeds irradiation on A549 cell is more remarkably than that of 60Co γ-rays.The imbalance of Bcl-2/Bax ratio and the eventually activation of Caspase-3 proteins may play an important role in the anti-proliferative effect induced by the continuous low dose radiation of 125I-seeds.

关 键 词：125I粒子 低剂量率 60Co 高剂量率 非小细胞肺癌 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤] R730.55[医药卫生—临床医学]