出生24小时内女婴阴道分泌物乳杆菌的研究  

Vaginal lactobacilli detection in baby girls within 24 hours ater birth

作　　者：韦晓昱[1] 肖冰冰[1] 廖秦平 

机构地区：[1]北京大学第一医院妇产科,北京100034 [2]北京清华长庚医院妇产科,北京102218

出　　处：《中国微生态学杂志》2015年第8期938-940,共3页Chinese Journal of Microecology

基　　金：2012年度高等学校博士学科点专项科研基金(新教师类)(20120001120037);国家自然科学基金青年基金(81200411);国家自然科学基金(81170535)

摘　　要：目的了解出生后24h内女婴阴道分泌物的乳杆菌带菌状态。方法选取2011年8月至2011年12月在北京大学第一医院足月分娩的女婴,剖宫产和阴道分娩的足月女婴各30例,剖宫产中胎膜早破22例,宫内感染2例;阴道分娩中胎膜早破18例,宫内感染9例,于出生后24h内取女婴阴道分泌物,分别行涂片革兰染色镜检及提取DNA使用乳杆菌属特异性引物行PCR扩增,分析阴道菌群状况。结果所有样本涂片行革兰染色镜检均未见革兰阳性杆菌,提取DNA行PCR扩增,均未见到明显条带。结论生后24h的女婴阴道分泌物为无乳杆菌状态。Objective To learn the positivity rate of Lactobacilli in the vagina of baby girls within 24 hours after birth.Methods A total of 60full-term female newborns at the Obstetrics and Gynecology Department of Peking University First Hospital from August 2011 to December 2011 were enrolled in this study,including 30 cases of cesarean delivery and 30 cases of vaginal delivery.Among the 30 cases of cesarean delivery,there were 22 cases of premature rupture of membranes and 2cases of intrauterine infection;Among the 30 cases of vaginal delivery,there were 18 cases of premature rupture of membranes and 9cases of intrauterine infection.The vaginal secretion samples of the newborns were collected within 24 hours after birth.Gram staining and PCR amplification were used to analyze the vaginal bacterial flora.Results Gram staining results of all samples showed no gram-positive bacteria.No obvious DNA band was discerned in PCR amplification.Conclusion There is no lactobacilli in the vaginal secretion of baby girls within 24 hours after birth.

关 键 词：女婴 阴道分泌物 乳杆菌 

分 类 号：R378.992[医药卫生—病原生物学]