喀斯特石生反叶扭口藓活性氧代谢对干旱胁迫的动态响应  

Dynamic Responses of Active Oxygen Metabolism of Barbula fallax in Rock Desertification of Karst Areas to Drought Stress

作　　者：张显强[1,2,3] 王世杰[1] 孙敏[3] 

机构地区：[1]中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550002 [2]安顺学院,安顺561000 [3]西南大学生命科学学院/三峡库区生态环境与生物资源省部共建国家重点实验室,重庆400715

出　　处：《基因组学与应用生物学》2015年第1期160-164,共5页Genomics and Applied Biology

基　　金：国家自然科学基金项目(41463006);国家973计划项目(2013CB956700)共同资助

摘　　要：本文研究了喀斯特石面常见植物反叶扭口藓(Barbula fallax Hedw.)在干旱胁迫下活性氧代谢的变化。结果显示:早期干旱超氧化酶歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性以及类胡萝卜素(Car)活性逐渐升高,胁迫后期活性下降;超氧阴离子(O-2.)、丙二醛(MDA)和可溶性蛋白含量呈现出先升后降的趋势;质膜相对透性呈现出"抛物线"的变化。干旱胁迫早期由于O-2等活性氧的增加而启动活性氧清除系统进行清除,是抵御干旱的一种协同反应;后期反叶扭口藓依然保持较强的自由基清除能力,具有极强的耐旱能力。In this research we studied the antioxidant protection mechanism of B.fallax in rock desertification of Karst areas under the conditions of drought stress simulated by PEG-6000.The result showed that the contents of B.fallax superoxide dismutase,catalase,peroxidase activity and carotenoids（Car） content increased in the early period of treatment,and declined late.When osmotic stress intensified,the B.fallax superoxide anion（O-2）,malondialdehyde（MDA） and soluble protein content exhibited the trend from going up and then slow down.As for the relative permeability of membrane were shown a parabola trend of.Therefore,the increases of early O-2and other reactive oxygen species（ROS） in drought stress might induce enzymatic scavenging system to start up clearance of ROS,which would be a synergistic reaction to resist drought.The B.fallax remained strong free radical scavenging capacity,leading to very strong resistant ability to drought.

关 键 词：喀斯特石漠化 反叶扭口藓 活性氧代谢 干旱胁迫 

分 类 号：Q945.78[生物学—植物学]