人参多糖注射液对人乳腺癌 MCF －7细胞侵袭迁移能力的影响及机制研究  

The effect and mechanism of ginseng polysaccharides injective solution on invasive ability of human breast cancer MCF-7 cells

作　　者：罗莹[1] 黄晶[2] 

机构地区：[1]湖北省松滋市人民医院,松滋434200 [2]武汉协和医院肿瘤中心

出　　处：《实用肿瘤学杂志》2015年第4期333-336,共4页Practical Oncology Journal

摘　　要：目的：探讨人参多糖注射液对人乳腺癌细胞株MCF－7侵袭迁移能力的影响。方法以不同作用浓度（12．5μL／mL、25μL／mL、50μL／mL、100μL／mL）人参多糖注射液处理MCF－7细胞株，以PBS作为空白对照，分别采用Transwell小室侵袭实验、划痕实验及Western blot检测细胞侵袭能力、迁移能力及基质金属蛋白酶－2（MMP－2）和基质金属蛋白酶－9（MMP－9）表达。结果人参多糖注射液对MCF－7细胞体外侵袭和迁移能力具有显著的抑制作用，并呈现剂量及时间依赖性。人参多糖注射液处理后，MCF－7细胞MMP－2蛋白表达及MMP－9蛋白表达比值显著降低，并呈现剂量及时间依赖性。结论人参多糖注射液可显著抑制人乳腺癌细胞MCF－7侵袭能力和迁移能力，下调MMP－2及MMP－9蛋白表达是其可能的作用机制。Objective To evaluate the effect and mechanism of ginseng polysaccharides injective solu-tion on invasive ability of human breast cancer MCF-7 cells.Methods Human breast cancer MCF-7 cells were treated with different concentrations of ginseng polysaccharides injections（12.5μL/mL,25μL/mL,50μL/mL or 100μL/mL）,and phosphate buffer saline was served as control.The invasive ability,transfer ability and protein expression of matrix metalloproteinases-2 （ MMP-2 ） and matrix metalloproteinases-9 （ MMP-9 ） were determined via transwell matrigel invasive test,scratch test and Western Blot,respectively.Results Ginseng pol-ysaccharide injection on invasive ability and transfer ability in vitro of MCF-7 cell had significant role in inhibi-ting effect,and showed dose and time dependent.After ginseng polysaccharides injection treatment,the MMP-2 and MMP-9 protein expression significantly reduced,and showed dose and time dependent manner.Conclusion Ginseng polysaccharides injection is able to inhibit the invasion and transfer in MCF -7 cells.Moreover,theregulation of MMP -2 and MMP -9 expression may be involved in this effect.

关 键 词：人参多糖注射液 乳腺癌 基质金属蛋白酶-2 基质金属蛋白酶-9 

分 类 号：R737.9[医药卫生—肿瘤]