Goldeneye^(TM) DNA身份鉴定系统22NC试剂盒的法医遗传学调查  

Forensic Investigation of Goldeneye^(TM) DNA ID 22NC Kit

作　　者：林源[1] 阙庭志[1] 赵珍敏[1] 张素华[1] 

机构地区：[1]司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063

出　　处：《法医学杂志》2015年第4期280-283,共4页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81302620);国家"十二五"科技支撑计划项目(2012BAK16B01)

摘　　要：目的调查Goldeneye TM DNA身份鉴定系统22NC试剂盒中所包含的21个常染色体STR基因座在汉族人群中的遗传学数据,并考察该试剂盒的法医学应用价值。方法应用Goldeneye TM DNA身份鉴定系统22NC试剂盒对华东地区500名汉族健康无关个体进行常染色体STR基因座分型检测,统计分析21个常染色体STR基因座的频率数据、群体遗传学参数及连锁不平衡状况。结果 21个常染色体STR基因座在华东汉族人群中均符合Hardy-Weinberg平衡,各基因座之间相互独立。DP值均大于0.85,在群体中的CDP值为1-3.616 5×10-26,二联体累积非父排除率为1-2.786 81×10-6,三联体累积非父排除率为1-8.545 82×10-10。结论Goldeneye TM DNA身份鉴定系统22NC试剂盒中所包含的21个常染色体STR基因座在华东汉族人群中具有良好的多态性,联合Goldeneye TM DNA身份鉴定系统20A可以满足双亲皆无的全同胞鉴定要求,为该类案件的解决提供便利的工具。Objective To investigate the genetic data of 21 autosom al STR included in G oldeneyeTM DNA ID 22N CK it in Chinese H an nationality and to evaluate the forensic application. Methods B y detected 500 unrelated healthy individuals in Chinese H an nationality of East China w ith G oldeneyeTM DNA ID 22N CK it, allele frequencies, population genetics param eters and linkage disequilibrium inform ation of the 21 autosom al STR w ere statistically analyzed. Results In the 21 autosom al STR , no deviations from H ardy-W einberg equilibrium w ere detected and all loci w ere independent form each other. D P values of 21 au-tosom al STR w ere all above 0.85, and the com bined discrim ination pow er w as 1-3.616 5×10^-26. Com bined m ean exclusion chance of this system in duo cases w as 1-2.786 81×10^-6, in trio cases w as 1-8.545 82× 10^-10. Conclusion Tw enty-one autosom al STR included in G oldeneyeTM DNA ID 22N CK it are highly polym orphic in the H an nationality. Com bined w ith G oldeneyeTM DNA ID 20A K it, the kit can satisfy the needs for full-sibling testing and facilitate the solution of this kind of case tools.

关 键 词：法医遗传学 多态现象 遗传 短串联重复序列 GoldeneyeTM DNA身份鉴定系统22NC试剂盒 

分 类 号：DF795.2[医药卫生—法医学]