板栗种子淀粉体发育的扫描电镜观察  

Observation of amyloplast development in chestnut seed by scanning electron microscope

作　　者：刘帅 陈良珂[1] 房克凤[1] 杨瑞[1] 邢宇[1] 曹庆芹[2] 秦岭[1] 

机构地区：[1]农业应用新技术北京市重点实验室.北京农学院植物科学技术学院,北京102206 [2]北京农学院生物科学与工程学院,北京102206

出　　处：《电子显微学报》2015年第4期346-350,共5页Journal of Chinese Electron Microscopy Society

基　　金：北京市属高等学校创新团队建设与教师职业发展计划项目(No.IDHT20140509);板栗种质创新研究创新平台建设资助项目(No.PXM2014_014207_000023)

摘　　要：为了探明板栗种子淀粉体生长的规律,以不同发育时期的板栗种子为试验材料,对淀粉体的大小、形态和发育情况等特性进行扫描电镜观察。结果表明:板栗糊粉层细胞中无淀粉体,不积累淀粉,淀粉体主要集中在子叶中;板栗种子有大、小两种淀粉体,大淀粉体呈鹅卵石形,小淀粉体呈圆球形;板栗种子以大淀粉体为主,空间排布较为紧密,此外,板栗淀粉体具有多面体形、球形、肾形、复合形等形态;板栗大淀粉体在种子发育前期和中期体积变化比较明显,而小淀粉体分布于大淀粉体的间隙中,增殖主要发生在种子发育中期和后期;淀粉体体积随发育天数的增加逐渐增大,长轴和短轴增长的变化呈"S"型生长曲线。In order to get the growth law of the Chinese chestnut seed amyloid,the chestnut seeds at different development stages were used as the experiment materials. The size,shape and development condition of amyloid features were observed by scanning electron microscopy. Results show that the aleurone layer cells have no amyloid and accumulation of starch. The amyloid mainly concentrates in the cotyledons. The chestnut seeds contain two kinds of amyliod： the large are pebble and the small are round. The large amyloid is the main kind,and its spatial arrangement is close. In addition,the amyliod has other forms in chestnut seeds,such as polyhedral,spherical,kidney,and complex form. The volume of large amyloid changes obviously in the prophase and metaphase stages in chestnut seeds. The small amyloplast proliferation mainly occurres in middle and late stages of seed growth,and distributes in clearance of large amyloid. As the chestnut seed develops,the volume of amyloid gradually increases,and the growth change of long axis and short axis shows ＂S＂type in the growing curves.

关 键 词：板栗 淀粉体 扫描电镜 

分 类 号：S664.2[农业科学—果树学] TS235.4[农业科学—园艺学]