大黄鱼内脏白点病的病原分析与鉴定  

Identification of Pathogen Responsible for White-spots Disease in Larimichthys crocea

作　　者：许斌福[1] 程海华 池洪树[1] 方冬兰[1] 柯巧珍 韩坤煌 王凡 龚晖[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003 [2]福建省宁德市富发水产有限公司,福建宁德352000 [3]福建省水产技术推广站,福建福州350003

出　　处：《福建农业学报》2015年第7期631-635,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目(2014N0010);福建省农业科学院科研项目(工程化实验室类)(2012DG-5);福建省财政专项--福建省农业科学院科技创新团队建设项目(STIT-Ⅱ-0321)

摘　　要：从福建三都澳网箱养殖患病的大黄鱼脾、肾分离到2株优势菌(H2013032002和H2013032003),通过人工感染试验证实其为大黄鱼内脏白点病病原,应用API-20E生理生化和16SrRNA分子系统鉴定,药物敏感试验筛选敏感药物。生化鉴定结果显示2株菌与恶臭假单胞菌和荧光假单胞菌的符合度为75.2%;16SrRNA序列分析结果表明:该2株菌与恶臭假单胞菌同源率高达99.0%,确定这2株菌为恶臭假单胞菌;药物敏感试验表明:病原菌对诺氟沙星、环丙沙星、恶喹酸、卡那霉素等4种药物高度敏感。Two strains of the suspected bacteria ,H2013032002 and H2013032003 ,were isolated from croakers , Larimichthys crocea ,in Sanduao ,Fujian .An artificial infection with the isolates caused the similar white‐spots disease in the fish .Subsequently ,morphological and biochemical tests identified the isolated strains as Pseudomonas putida or P . f luorescent . Then , the 16S‐rDNA sequence analysis showed that the bacteria had a 99.0%homologous rate with P .putida .In addition ,a drug sensitivity test revealed that the two strains of pathogen were highly sensitive to norfloxacin ,ciprofloxacin ,oxolinic acid and kanamycin neomycin .It was ,therefore ,determined that P .putid was likely the culprit that caused the white‐spots disease in croakers .

关 键 词：大黄鱼 内脏白点病 恶臭假单胞菌 16SrRNA 药物敏感 

分 类 号：S941.42[农业科学—水产养殖]