CCl_4诱导大鼠纤维化肝组织microRNA差异表达及其初步分析  

Differentially expressed and preliminary analysis of microRNA profiles in liver tissue of rat induced by carbon tetrachloride

作　　者：刘业方 张传涛[2] 李白雪[1] 郭尹玲[1] 王宝家[1] 唐洪屈 王政[1] 张凤[1] 杨宇[1] 

机构地区：[1]成都中医药大学基础医学院,成都610075 [2]成都中医药大学附属医院感染科,成都610072 [3]重庆市垫江县中医院肿瘤科,408300

出　　处：《重庆医学》2015年第25期3473-3476,3480,共5页Chongqing medicine

基　　金：国家自然科学基金资助项目(81373530);教育部博士点基金(20125132120011);四川省教育厅科研项目(132B0321);成都中医药大学校基金(ZRMS201230)

摘　　要：目的探索microRNA在四氯化碳(CCl4)诱导的大鼠纤维化肝组织中的差异表达及其意义。方法采用microRNA高通量测序分析技术检测对照组(n=10)与模型组(n=10)肝脏组织microRNA表达,对差异microRNA进行靶基因预测,对靶基因进行基因本(GO)分析、pathway分析。结果对照组与模型组间筛选出37个差异microRNA,上调29个,下调8个;从microRNA-基因网络图中发现关键上调为miR-184、miR-10b-5p、miR-199a-3p等;关键下调为miR-200b-3p、miR-199a-5p、miR-125b-5p等。结论模型组microRNA表达谱变化明显,肝纤维化的形成与microRNA调控细胞增殖、凋亡、细胞周期等有关。Objective To explore the differences of expression profiles and significance of the microRNA in liver tissue of rat induced by carbon tetraehloride（CCl4 ）. Methods The microRNA differential expression analysis between normal group（n = 10） and model group（n= 10） was conducted on high-throughput methods. Gene ontology enrichment analysis and kyoto encyclopedia of genes and genomes pathway analysis of the targets of miRNA were carried out in this study. Results Some differentally expressed microRNA with 29 up-regulated and 8 down-regulated were identified in our Study. Through evaluaing the value of microRNA in the network, we find that the key microRNA of rising are miR-184,miR-10b-5p, miR-199a-3p,and the key microRNA of cutting are miR-200b-3p,miR-199a-5p,miR-125b-5p and so on. Conclusion The microRNA expression level in the model group has a signifi- cant change. Therefore,the courses of liver fibrosis is related to some process which microRNA control the regulation of cell proliferation, cell apoptosis, cell cycle,and so on.

关 键 词：微小RNA 肝纤维化 四氯化碳 差异表达 

分 类 号：R575.2[医药卫生—消化系统]