Id2和MMP9的表达及其与食管鳞癌的临床病理特征关系研究  

Expression of Id2 and MMP9 in esophageal squamous cell carcinoma and its relationship with clinicopathological features

作　　者：岳黎敏[1] 程书珍[2] 郑海萍[3] 商小涓[1] 

机构地区：[1]河北工程大学医学院基础医学系,河北邯郸056029 [2]河北工程大学医学院检验系,河北邯郸056029 [3]河北工程大学附属医院中心实验室,河北邯郸056029

出　　处：《重庆医学》2015年第25期3503-3505,共3页Chongqing medicine

基　　金：河北省科技计划项目基金资助(122077176D)

摘　　要：目的观察细胞分化/DNA结合抑制因子2(Id2)和基质金属蛋白酶9(MMP9)在食管鳞癌组织中的表达,探讨Id2和MMP9表达的意义及相关性。方法应用免疫组织化学方法检测70例食管鳞癌和30例正常食管上皮中Id2和MMP9的表达水平。结果食管鳞癌中Id2和MMP9的阳性表达率均显著高于正常食管上皮(P<0.01);Id2和MMP9的表达水平均随食管鳞癌的浸润加深、临床TNM分期级别增高而升高,浸润深度在T3+T4组的表达水平高于T1+T2组(P<0.05),临床Ⅱ期和Ⅲ、Ⅳ期的表达水平高于Ⅰ期(P<0.05),但Id2和MMP9的表达均与食管鳞癌的分化程度无显著相关(P>0.05)。淋巴结转移组中MMP9的表达水平高于无淋巴结转移组(P<0.05);而Id2的表达与淋巴结转移无显著相关(P>0.05);食管鳞癌组织中Id2和MMP9的表达水平呈正相关关系(P<0.05)。结论 Id2和MMP9在食管鳞癌的发生和浸润进展中存在协同促进作用,同时检测对于评估食管鳞癌的生物学行为和预后有重要意义。Objective To investigate the expression and ciinicopathological significance of inhibitors of differentiation 2（Id2） and Matrix metalloproteinaseg（MMPg）in esophageal squamous cell carcinoma（ESCC） and explore their correlation. Methods The expression of Id2 and MMP9 in 70 cases of ESCC and 30 cases of controls were detected by immunohistochemical staining. Results The positive rates of Id2 and MMP9 in ESCC were significantly higher than those in normal esophageal epithelia （P〈0.01）. Expression levels of Id2 and MMP9 were increased as the infiltration depth and TNM staging increased,and the levels were higher in T3 ＋T4 group and II , III, IV stage than in T1 ＋T2 group and I stage（P〈0.05）, but not correlate to pathologic grades （P〉0.05）. Expression level of MMP9 was higher in lymph node metastasis group than in group without lymph node metastasis （P〈0.05） ,but expression levels of Id2 were not related to lymph node metastasis（P〉0.05）. The expression of Id2 and MMP9 were positively correlated（P〈0.05）. Conclusion Id2 and MMP9 might synergistically promote the tumorigenesis and development of ESCC,and their co-detection can he of great importance for estimation of biological behavior and prognosis of ESCC.

关 键 词：食管肿瘤 ID2 基质金属蛋白酶9 

分 类 号：R735.1[医药卫生—肿瘤]