芡实根际促生细菌的筛选及其生物学特性  

Screening of Growth-Promoting Bacteria from Euryale ferox Rhizosphere and Their Biological Characteristics

作　　者：张义[1] 程晓 王全龙 刘淑杰[1] 吴秉奇[1] 

机构地区：[1]深圳清华大学研究院/深圳环境微生物资源开发与应用工程实验室,广东深圳518057 [2]苏州澄湖现代科技生态农业发展有限责任公司,江苏苏州215127

出　　处：《山东农业科学》2015年第8期53-58,共6页Shandong Agricultural Sciences

基　　金：苏州市科技计划农业科技支撑计划(SNG201329)

摘　　要：为给水生植物芡实(Euryale ferox)生物肥料的研制提供优良的菌株资源,采用保绿法和萝卜子叶增重法从芡实根际土壤筛选出2株作用效果较好的细菌Q6-2和Q67,用高效液相色谱法(High Performance Liquid Chromatography,HPLC)定量分析菌株产植物激素和有机酸的能力;通过盆栽试验研究2株细菌对苗期芡实的促生作用;结合生理生化试验和16S r DNA序列分析对2株细菌进行鉴定。结果显示,2株细菌均能够产生玉米素、激动素等植物激素和少量有机酸,Q6-2还能够产生吲哚乙酸;接种Q6-2后,芡实幼苗茎长、叶片数、最大叶面积、生物量(干重)分别比对照高出18.85%、40.67%、76.28%和37.50%,接种Q67的处理各项指标分别比对照高出27.47%、42.81%、96.05%和42.97%,差异显著;接种Q6-2和Q67后,叶绿素含量分别高出对照43.27%和51.46%,根系活力则分别高出5.41%和7.05%,差异显著。经鉴定,Q6-2和Q67均为地衣芽孢杆菌,Gen Bank登录号分别为KP686129和KP686133。2株细菌有效促进了芡实幼苗生长发育水平,在微生物肥料的生产中具有广阔的应用前景。This study aims to provide excellent bacterial strain resources for the production of Euryale ferox bio- fertilizer. Two strains with relatively higher performance named Q6- 2 and Q67 were screened from the rhizosphere soil of Euryale ferox using the methods of remaining green and radish cotyledon bioassay; their production capability of plant hormones and organic acids were quantitatively analyzed by HPLC; their promotion effects on the growth of Euryale ferox at seedling stage were investigated by pot experiments; also,they were identified by physiological and biochemical experiments and 16 S r DNA sequence analysis. The results indicated that the two strains were both able to produce zeatin,kinetin and small amount of organic acid. Q6- 2was also able to produce indoleacetic acid（ IAA）. After inoculated by Q6- 2,the growth parameters of Euryale ferox seedlings,such as stem length,number of leaves,maximum leaf area and biomass（ dry weight）,significantly increased by 18. 85%,40. 67%,76. 28% and 37. 50% respectively compared to the control;while inoculated by Q67,the above mentioned indexes significantly increased by 27. 47%,42. 81%,96. 05% and 42. 97% respectively. After inoculated by Q6- 2 and Q67,the chlorophyll content increased by43. 27% and 51. 46% respectively,and the root activity increased by 5. 41% and 7. 05% respectively,showing significant differences compared to the control. Q6- 2 and Q67 were both identified as Bacillus licheniformis,whose Gen Bank accession number were KP686129 and KP686133 respectively. In conclusion,the two strains were able to promote the growth and development of Euryale ferox seedlings efficiently,showing great application prospect in the production of microbial fertilizer.

关 键 词：芡实 植物根际促生菌 植物激素 促生 鉴定 

分 类 号：S154.381[农业科学—土壤学]