葛根素对α-葡萄糖苷酶抑制作用机理的探讨  

Study of the inhibitive mechanism of puerarin on α-glucosidase

作　　者：徐婷[1] 褚江洪 丁萍英[1] 倪连松[1] 

机构地区：[1]温州医科大学附属台州医院内分泌科,浙江临海317000

出　　处：《中国生化药物杂志》2015年第1期43-45,48,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：浙江省医药卫生科技计划项目(2011KYA160)

摘　　要：目的探讨葛根素对α-葡萄糖苷酶的抑制作用机理。方法取40只雄性小鼠,分为5组:对照组、阿卡波糖对照组、葛根素(2.5、5.0、10)μM,每组8只,采用酶抑制动力学方法,研究葛根素对α-葡萄糖苷酶的抑制作用和抑制动力学,并用分光光度计测定葛根素对小鼠血糖含量变化的影响。结果葛根素是一种可逆的竞争型α-葡萄糖苷酶抑制剂[半抑制浓度IC50为(4.32±0.42)μM],抑制常数Ki为(0.41±0.09)μM;与阳性对照阿卡波糖对比,葛根素能够明显的抑制大鼠血糖水平的增长。结论葛根素作为一个α-葡萄糖苷酶抑制剂,诱导了酶活性的降低,对调节血糖水平起到重要作用,为临床降血糖提供了理论基础。Objective To study the inhibitory mechanism of puerarin on α-glucosidase. Methods 40 male mices were divided into 5 groups：control group,acarbose group,puerarin（ 2. 5,5. 0,10） μM group,8 mices in each group. Studied the inhibitory effect and inhibition kinetics of puerarin on α-glucosidase by enzyme inhibition kinetics method,and spectrophotometer was used to determine the effect of puerarin on blood glucose change in mices. Results Puerarin was a reversible competitive α-glucosidase inhibitor,the value half inhibition concentration（ IC50） and inhibition constant（ Ki）were obtained to be（ 4. 32 ± 0. 42） μM and（ 0. 41 ± 0. 09） μM,respectively. Compared with acarbos,puerarin could inhibite the increase of the blood glucose levels. Conclusion Puerarin as a competitive α-glucosidase inhibitor,can reduce the enzyme actiuity,and play an important role in fall blood ylucose level.

关 键 词：葛根素 Α-葡萄糖苷酶 抑制作用 血糖水平 糖尿病 

分 类 号：R285[医药卫生—中药学]