八种常用化疗药物组合对人类宫颈癌细胞体外药物敏感试验研究  

Study on susceptibility of eight common chemotherapy drug combinations in human cervical carcinoma cell in vitro

作　　者：周影[1] 胡建铭[1] 

机构地区：[1]苏州大学附属第一医院妇产科,苏州215006

出　　处：《中国生化药物杂志》2015年第1期57-59,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的探讨临床宫颈癌原代肿瘤细胞对8种常用化疗药物组合方案的敏感性。方法 2013年6月~2014年10月苏州大学附属第一医院收治的41例宫颈癌术中切下肿瘤组织标本进行体外细胞培养,再将紫杉醇,紫杉醇＋顺铂,紫杉醇＋卡铂,托泊替康＋顺铂,吉西他滨＋顺铂,多西他赛,吉西他滨以及顺铂＋环磷酰胺＋多柔比星分别设定不同药物浓度梯度[200%,100%,50.0%,25.0%,12.5%血浆峰值浓度（plasma peak concertration,PPC）],采用ATP生物荧光法进行药敏试验。结果 8组化疗药物方案血浆峰值浓度下对宫颈癌的敏感性依次为：紫杉醇＋顺铂〉紫杉醇＋卡铂〉紫杉醇〉吉西他滨＋顺铂〉顺铂＋环磷酰胺＋多柔比星〉托泊替康＋顺铂〉多西他赛〉吉西他滨。结论宫颈癌患者进行化疗药物敏感试验具有可行性,对临床个体化化疗具有现实指导作用。Objective To evalute chemosensivtivity of cervical pro-genetation turercells to eight common chemotherapy dnig combinations.Methods 41 fresh specimens of human cervical tumors were selected in the first affiliated hospital of Soochow university from June 2013 to October2014,then the cells were cultured. The sensitivity of combinations were tested using ATP-bioluminesence method： taxol（ TAX）,TAX ＋ cisplatin（ DDP）,TAX ＋ carboplatin（ CBP）,topotecan ＋ DDP,gemcitabine（ GEM） ＋ DDP,docetaxel,GEM and DDP ＋ cyclophosphamide（ CTX） ＋ doxoru（ ADM） in different concentrations[200%,100%,50. 0%,25. 0%,12. 5% plasma peak concentration（ PPC） ]. Results The chemosensitivities priority of cervical carcinoma to eight anticancer drugs in plasma peak concentration： TAX ＋ DDP TAX ＋ CBP TAX GEM ＋ DDP DDP ＋ CTX ＋ADM Topotecan ＋ DDP Docetaxel GEM. Conclusion The ATP-bioluminesence assay in vitro can predict chemotherapy for cervical carcinoma,which has practical guidance for individual chemotherapy.

关 键 词：宫颈癌 原代肿瘤细胞 化疗药物 敏感性 体外培养 

分 类 号：R737.3[医药卫生—肿瘤]