大蒜素对大鼠心肌缺血的保护作用及相关机制研究  

Protective effects of allicin on myocardial ischemia in rats and related mechanisms

作　　者：展宏刚 孙淑娟[2] 梁孝印[3] 程梅[4] 

机构地区：[1]山东省泰山疗养院药剂科,山东泰安271000 [2]山东省千佛山医院药剂科,山东济南250014 [3]泰安市中心医院,山东泰安271000 [4]山东大学临床医学系,山东济南250100

出　　处：《中国生化药物杂志》2015年第1期74-76,80,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：2008年度山东省自然科学基金项目(Y2008C100)

摘　　要：目的探究大蒜素对大鼠心肌缺血的保护作用及其相关机制。方法将50只准性SD大鼠随机平均分成假手术组(SO组),缺血/再灌注组(I/R组),大蒜素处理组高剂量组(HA组)、中剂量组(MA组)、低剂量组(LA组),每组10只。大蒜素处理组分别灌胃给予100、50、25 mg/(kg·d)大蒜素,给药20 d后构建大鼠心肌缺血/再灌注模型。检测各组大鼠血清及缺血心肌组织肌酸激酶同工酶(creatine kinase-MB,CK-MB),丙二醛(malondialdehyde,MDA),超氧化物歧化酶(superoxide dismutase,SOD)含量;Tunel法检测各组大鼠心肌组织细胞凋亡情况;免疫印迹法检测天冬氨酸蛋白水解酶-3(aspartic protease-3,caspase-3),磷酸化分裂原激活的蛋白激酶(phosphorylation of mitogen activated protein kinase,p-p38MAPK)及分裂原激活的蛋白激酶(mitogen-activated protein kinase,p38MAPK)的表达情况。结果 HA组与MA组的CK-MB、MDA水平均比I/R组显著偏低(P<0.05),HA组的SOD水平较I/R组显著偏高(P=0.015)。HA组,MA组的心肌损伤范围及凋亡指数较I/R组显著偏低(P<0.05),HA组caspase-3蛋白表达水平较I/R组显著偏低(P=0.023)。5组p38MAPK蛋白表达量无显著差异,但是HA组p-p38MAPK蛋白表达量较I/R组显著偏高(P=0.038)。结论大蒜素对大鼠心肌缺血具有明显的保护作用,且这种保护作用可能与心肌凋亡的抑制作用及p38蛋白的活化具有一定关系。Objective To investigate the protective effect of allicin on myocardial ischemia in rats and related mechanisms. Methods 50 SD rats were randomly divided into sham operation group（ SO group）,ischemia / reperfusion group（ I / R group）,allicin treatment groups： high dose allicin group（ HA group）,middle dose allicin group（ MA group）,low dose allicin group（ LA group）,10 rats in each group. 100,50,25 mg /（ kg·d） allicin were administered into rats in allicin treatment groups for 20 days,respectively. All groups except SO group were constructed of myocardial ischemia /reperfusion（ I / R） model. Serum and ischemic myocardial tissue were obtained to examine creatine kinase-MB（ CK-MB）,malondialdehyde（ MDA）,and superoxide dismutase（ SOD） by the corresponding kit; cardiac tissue were used to detect damage extents and cell apoptosis by Tunel method;myocardial protein was extracted and the expression of aspartic protease-3（ caspase-3）, phosphorylation of mitogen activated protein kinase（ p-p38MAPK） and mitogen-activated protein kinase（ p38MAPK） were detected by Western blot. Results Levels of CK-MB and MDA in HA group and MA group were lower than I / R group（ P〈0. 05）,and level of SOD was higher（ P = 0. 015）. Myocardial injury extent and apoptosis index in HA group and MA group were smaller than that in I / R group（ P〈0. 05）. Caspase-3 protein expression level in HA group was lower than that in I / R group（ P = 0. 023）. No significant difference was detected among the five groups in p38 MAPK protein expression,but HA group p-p38 MAPK protein expression level was significantly higher than I / R group（ P = 0. 038）. Conclusion Allicin has a significant protective effect on myocardial ischemia,and may have some relationship with the inhibition of myocardial apoptosis and p38 MAPK activation.

关 键 词：大蒜素 心肌缺血 凋亡 I/R P38MAPK 

分 类 号：R285.5[医药卫生—中药学]