维生素D受体基因多态性与慢性牙周炎的相关性  

Relationship between vitamin D receptor gene polymorphisms and chronic periodontitis

作　　者：曹晓静[1,2] 和璐[1] 孟焕新[1] 李蓬[1,3] 陈智滨[1] 

机构地区：[1]北京大学口腔医学院.口腔医院牙周科,北京100081 [2]北京大学口腔医院门诊部综合科 [3]北京大学口腔医院第二门诊部牙周科

出　　处：《北京大学学报（医学版）》2015年第4期697-702,共6页Journal of Peking University:Health Sciences

基　　金："十一五"国家科技支撑计划重点项目(2007BAI18B02);卫生部临床重点学科项目(2010-2012)资助~~

摘　　要：目的:探讨中国汉族维生素D受体(vitamin D receptor,VDR)基因多态性与慢性牙周炎的关系。方法:选取105例轻/中度慢性牙周炎和85例重度慢性牙周炎患者,采集口腔颊黏膜拭子,提取DNA,应用基质辅助激光解吸电离飞行时间质谱技术(matrix-assisted laser desorption ionization time-of-flight,MALDI-TOF)检测VDR基因的多态性。结果:在受检总人群及女性患者中,rs1544410的A等位基因及AA+AG基因型在重度牙周炎组检出率高于轻/中度组(总人群:P=0.006,0.007;女性患者:P=0.001,0.001);rs731236的C等位基因及CC+CT基因型在重度牙周炎组检出率高于轻/中度组(总人群:P=0.003,0.004;女性患者:P<0.001,<0.001)。结论:VDR的rs731236和rs1544410基因多态性可能是中国汉族重度慢性牙周炎的危险因素,VDR多态性对牙周炎程度的影响可能存在性别差异。Objective： To investigate the relationship between vitamin D receptor（ VDR） gene polymorphisms and chronic periodontitis（ CP）. Methods： Buccal swabs from 105 patients with mild / moderate CP and 85 severe CP were collected,DNA was extracted from these buccal swabs using the TIANamp Swab DNA Kit [TIANGEN Biotech（ Beijing） CO. Ltd]. The VDR rs1544410 and rs731236 were genotyped by the Sequenom Mass ARRAY system（ Shanghai Benegene Biotechnology Co. Ltd）,which was based on MALDI-TOF（ matrix-assisted laser desorption ionization time-of-flight） technology. The distribution of the genotypes and allele frequencies were analyzed. Results： The frequencies of the rs1544410 A allele and AA ＋ AG genotype were significantly higher in severe CP than in mild / moderate CP of all the patients and the female patients respectively（ all the patients： P = 0. 006,0. 007; the female patients： P = 0. 001,0. 001）. The frequencies of the rs731236 C allele and CC ＋ CT genotype were significantly higher in severe CP than in mild / moderate CP of all the patients and the female patients respectively（ all the patients： P =0. 003,0. 004; the female patients： P〈0. 001,0. 001）. Conclusion： Gene polymorphisms of VDR rs731236 and rs1544410 may be associated with severe CP in Chinese Han population.

关 键 词：牙周炎 受体 维生素D 多态现象 遗传 

分 类 号：R781.42[医药卫生—口腔医学]