缺氧调控人肝癌细胞核转录因子NF-κBp65/基质金属蛋白酶9表达相关机制的研究  

Expression mechanisms of hypoxic regulation of NF-κBp65/MMP-9 in human hepatocellular carcinoma cells

作　　者：肖路[1] 薛同敏[1] 王敏[1] 刘伟[1] 张攀[1] 张培建[1] 

机构地区：[1]扬州大学第二临床医学院普通外科研究室,225002

出　　处：《中华普通外科学文献（电子版）》2015年第4期16-18,共3页Chinese Archives of General Surgery(Electronic Edition)

基　　金：江苏省扬州市社会科技计划面上基金资助项目(YZ2014064)

摘　　要：目的探讨缺氧调控人肝癌细胞（Hep G2）核转录因子NF-κBp65/基质金属蛋白酶9（MMP-9）表达相关机制。方法采用Co Cl2模拟低氧环境处理细胞,MTT法检测细胞的增殖,Western blotting法测定胞质蛋白NF-κBp65、MMP-9蛋白的表达。结果低氧处理后胞质NF-κBp65、MMP-9的活性增加,当Co Cl2浓度在50-200μmol/L时MMP-9表达呈现剂量依赖性,当Co Cl2浓度超过200μmol/L后,MMP-9的表达不再增加;而NF-κBp65的表达继续增加,并且细胞迁移侵袭能力增加与MMP-9的表达呈正相关。结论一定的低氧环境下肝癌细胞（Hep G2）NF-κBp65、MMP-9的表达增加,并且细胞迁移侵袭能力增加与MMP-9的表达呈正相关。Objective To investigate the expression mechanisms of hypoxic regulation of NF-κBp65/MMP-9 in human hepatocellular carcinoma cells（Hep G2）. Methods Hep G2 were induced by Co Cl2, and MTT assay was used for detection of cellular proliferation. The expression of NF- κBp65/MMP-9 in cytosolic proteins was determined by Western blotting. Results After hypoxic treatment, the activity of cytoplasmic NF-κBp65/MMP-9 increased when the concentration of Co Cl2 was between 50-200 μmol/L, while MMP-9 expression was dose-dependent. When the concentration of Co Cl2 exceeded 200 μmol/L,MMP-9 expression was not obvious, while NF-κBp65 expression increased with the concentration of Co Cl2.The migration and invasion capacity of cells positively correlated with MMP-9 expression. Conclusion NF-κBp65 and MMP-9 expressions of Hep G2 increase under certain hypoxic environment, and the increased cell migration and invasion positively correlate to the expression of MMP-9.

关 键 词：肝癌 低氧 NF-ΚBP65 基质金属蛋白酶9 

分 类 号：R735.7[医药卫生—肿瘤]