血管内皮生长因子及其磷酸化受体在肝外胆管细胞癌中表达的研究  

Clinical significance of VEGF and its phosphorylated receptors in extrahepatic cholangiocarcinoma

作　　者：陈惠新[1] 陈丽莎[1] 徐永成[1] 余志金[1] 程进伟[1] 曾书君[1] 

机构地区：[1]广东省惠州市中心人民医院消化内科,516000

出　　处：《中华普通外科学文献（电子版）》2015年第4期19-22,共4页Chinese Archives of General Surgery(Electronic Edition)

摘　　要：目的探讨血管内皮生长因子（VEGF）及其磷酸化受体表达在肝外胆管细胞癌（EHCC）中的临床意义。方法免疫组化法检测VEGF及其磷酸化受体p VEGFR1/2在35例EHCC患者组织中的表达情况,Western blotting检测EHCC细胞系中VEGF及其相关受体的表达情况,探讨VEGF及其受体蛋白的表达与肿瘤进展、预后的相关性。结果 VEGF、p VEGFR1和p VEGFR2阳性的病例数分别为20（57.1%）、14（40.0%）和14（40.0%）例。p VEGFR1的高表达与细胞增殖相关蛋白p Erk1/2水平、神经侵犯、TNM分期、肿瘤分化程度、静脉侵犯程度均相关（P均小于0.05）。p VEGFR1的高表达增加EHCC患者的死亡风险（HR=4.45,95%CI 38.66-61.62,P=0.035）。结论p VEGFR1/2的表达程度与EHCC的进展呈正相关,高表达增加患者死亡风险,提示p VEGFR1/2有可能作为EHCC患者临床预后的预测因子。Objective To study the clinical significance of vascular endothelial growth factor（VEGF） and its phosphorylated receptors in extrahepatic cholangiocarcinoma（EHCC）. Methods Immunohistochemistry and Western blotting were used to determine the expression of VEGF and phosphorylated VEGF receptors（p VEGFR1/2） in EHCC tissues or EHCC cell lines of thirty-five cases. Clinical data were collected and further analyzed with protein expression of VEGF and p VEGFR1/2. Results There were 20（57.1%）, 14（40.0%） and 14（40.0 %） positive cases of VEGF, p VEGFR1 and p VEGFR2, respectively. The over-expression of p VEGFR1 was correlated with p Erk1/2, perineural invasion, TNM staging, tumor differentiation and vascular invasion（P〈0.05）. Over-expression of p VEGFR1 showed poor overall survival of EHCC patients（HR=4.45, 95% CI： 38.66-61.62, P=0.035）. Conclusion In EHCC, p VEGFR1 and p VEGFR2 are correlated with tumor progression and poor prognosis, indicating that VEGF and its receptors may serve as predicting factors for prognosis in EHCC.

关 键 词：血管内皮生长因子 血管内皮生长因子受体 肝外胆管细胞癌 预后 

分 类 号：R735.8[医药卫生—肿瘤]