Ezrin蛋白通过上调YAP的表达促进胃癌的侵袭转移  

Ezrin promotes invasion and metastasis of gastric cancer by up-regulating YAP protein expression

作　　者：单汉国[1] 黄昌浩[1] 黄笛鸣[1,2] 

机构地区：[1]中南大学湘雅医院胃肠外科,湖南省长沙市410013 [2]南华大学附属第二医院胃肠外科,湖南省衡阳市421001

出　　处：《世界华人消化杂志》2015年第23期3683-3690,共8页World Chinese Journal of Digestology

摘　　要：目的:探讨Ezrin与Hippo通路下游蛋白YAP(Yes-associated protein)在胃癌侵袭转移中的相互作用机制.方法:免疫组织化学检测209例胃癌组织中的Ezrin和YAP的蛋白表达水平.MTT法检测过表达Ezrin及同时干扰YAP后对细胞增殖的影响.Transwell法检测过表达Ezrin及同时干扰YAP后对细胞迁移和侵袭的影响.结果:免疫组织化学结果表明Ezrin及YAP在胃癌组织中高表达,并与肿瘤的发生发展和转移相关,且两者的表达水平呈正相关的关系.MTT检测结果表明过表达Ezrin后胃癌细胞生长增殖能力增加,而同时干扰YAP后生长增殖能力受到抑制.Transwell结果表明过表达Ezrin后胃癌细胞迁移侵袭能力增加,而同时干扰YAP后迁移侵袭能力受到抑制.结论:Ezrin在胃癌中可能起类似癌基因的作用,其可能通过上调Hippo通路的下游蛋白YAP促进胃癌的发生发展与侵袭转移.AIM： To explore the interactions of Ezrin and Hippo/Yes-associated protein（YAP） in the invasion and metastasis of gastric cancer. METHODS： Immunohistochemistry（IHC）was used to determine the protein expression of Ezrin and YAP in 209 gastric cancer tissues. MTT assay was carried out to evaluate the effect of Ezrin overexpression with or without si YAP treatment on cell proliferation. Transwell assays were implemented to assess the impact of Ezrin overexpression with or without si YAP on cell migration and invasion. RESULTS： IHC staining showed that Ezrin and YAP expression was up-regulated in primary gastric cancer compared with their adjacent normal tissues and positively correlated with tumor occurrence, development and metastasis. MTT assay showed that overexpression of Ezrin increased cell proliferation, and knockdown of YAP inhibited cell proliferation. Transwell assays demonstrated that the ectopic expression of Ezrin in SGC7901 cells promoted cell migration and invasion, and knockdown of YAP inhibited cell migration and invasion.CONCLUSION： Ezrin functions as an oncogene in gastric cancer and can increase YAP protein expression, which in turn up-regulates the expression of the downstream molecules of the Hippo pathway to promote invasion and metastasis of gastric cancer.

关 键 词：EZRIN YAP 胃癌 侵袭 转移 

分 类 号：R735.2[医药卫生—肿瘤]