Effects of clenbuterol exposure on PPARγ expression in adipocytes of rats  

作　　者：Jing LI Wei-jie ZHU Bao-guo XIE 

机构地区：[1]Department of Pathophysiology, Medical College, Jinan University [2]Department of Developmental and Regenerative Biology, College of Life Science and Technology, Jinan University

出　　处：《Journal of Reproduction and Contraception》2015年第2期67-72,共6页生殖与避孕（英文版）

基　　金：supported by Science and Technology Planning Project of Guangzhou City,China(No.201300000114)

摘　　要：Objective To investigate effects of clenbuterol （CLB） on the peroxisome proliferators- activated receptor γ （PPARγ） expression in adipose tissues of rats. Methods Twenty adult female Sprague-Dawley rats were randomly divided into 4 groups （5 rats per group）. CLB solved in normal saline solution was given at the dose of 0 mg/kg body weight （bw） （group A, as the control）, 0.4 mg/kg bw （group B, low-dose group）, 2.0 mg/kg bw （group C, mid-dose group）, and 18.5 mg/kg bw （group D, high-dose group）for 14 d by gavage consecutively, respectively. Methods of immunohistochemistry, quantitative Real-time PCR and Western blotting were performed to detect expression of PPARγ in the adipose tissue samples. Results PPARγ-positive immunostaining was strong in the controls and weak in the experimental groups. There was no difference on PPARγmRNA and protein between the low-dose group and the control （P〉0.05）. With the increase of CLB doses, expression levels of PPARγmRNA and protein were significantly lower in mid- or high-dose group than those in the control （19〈0.01）. Conclusions The PPARγ expression in adipose tissues of rats could be down-regulated after CLB exposure, and the decrease became more severe with the increasing doses.Objective To investigate effects of clenbuterol （CLB） on the peroxisome proliferators- activated receptor γ （PPARγ） expression in adipose tissues of rats. Methods Twenty adult female Sprague-Dawley rats were randomly divided into 4 groups （5 rats per group）. CLB solved in normal saline solution was given at the dose of 0 mg/kg body weight （bw） （group A, as the control）, 0.4 mg/kg bw （group B, low-dose group）, 2.0 mg/kg bw （group C, mid-dose group）, and 18.5 mg/kg bw （group D, high-dose group）for 14 d by gavage consecutively, respectively. Methods of immunohistochemistry, quantitative Real-time PCR and Western blotting were performed to detect expression of PPARγ in the adipose tissue samples. Results PPARγ-positive immunostaining was strong in the controls and weak in the experimental groups. There was no difference on PPARγmRNA and protein between the low-dose group and the control （P〉0.05）. With the increase of CLB doses, expression levels of PPARγmRNA and protein were significantly lower in mid- or high-dose group than those in the control （19〈0.01）. Conclusions The PPARγ expression in adipose tissues of rats could be down-regulated after CLB exposure, and the decrease became more severe with the increasing doses.

关 键 词：clenbuterol （CLB） peroxisome proliferators-activated receptor γ （PPARγ） adipose tissue rat 

分 类 号：R363[医药卫生—病理学]