补肾活血中药对大鼠慢性高眼压模型视网膜神经节细胞PI3K/Akt信号转导通路p-Akt表达的影响  

Effects of Bushenhuoxue on expression of p-Akt of PI3K / Akt signal transduction pathway in RGC in rat model of chronic elevated intraocular pressure

作　　者：汪伟[1,2] 李翔[3] 王桃[4] 柯欣怡 刘红佶 贾正品 牟琳 

机构地区：[1]成都中医药大学,四川省成都市610072 [2]四川医科大学附属中医医院,四川省泸州市646000 [3]成都中医药大学附属医院,四川省成都市610072 [4]萍乡市人民医院,江西省萍乡市337000

出　　处：《眼科新进展》2015年第9期816-820,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:81373695)~~

摘　　要：目的观察补肾活血中药对大鼠慢性高眼压(elevated intraocular pressure,EIOP)模型视网膜神经节细胞(retinal ganglion cells,RGC)PI3K/Akt信号转导通路p-Akt表达的影响。方法 30只SD大鼠随机分成3组:对照组、模型组和给药组,模型组和给药组建立EIOP模型。给药组造模后每天予复方丹参片和杞菊地黄丸的混悬液灌胃,每天1次,连续8周,对照组、模型组每天予生理盐水灌胃。记录造模前、造模后即刻、造模后8周的眼压;光镜下观察各组视网膜厚度和RGC数量,电镜下观察视网膜超微结构,免疫组织化学法检测视网膜内p-Akt的表达。结果造模后8周眼压与造模后即刻相比,给药组有降低趋势,差异有统计学意义(P<0.01)。造模后8周,给药组与模型组RGC数量均低于对照组,差异均有统计学意义(均为P<0.05);对照组视网膜厚度与给药组相比,差异无统计学意义(P>0.05),而对照组与模型组相比,差异有统计学意义(P<0.01);给药组RGC数量及视网膜厚度均高于模型组,差异均有统计学意义(均为P<0.01)。电镜下RGC超微结构显示,模型组损伤明显,给药组较模型组有所改善。视网膜p-Akt表达免疫组织化学结果分析显示,给药组p-Akt阳性染色总面积、平均光密度、积分光密度值与模型组相比,差异均有统计学意义(均为P<0.01),给药组黑度(139.97±11.79)虽高于模型组(137.95±6.13),但差异无统计学意义(P>0.05)。结论补肾活血中药用于SD大鼠慢性EIOP模型,能降低眼压,提高RGC数量及改善超微结构,增加视网膜厚度,上调PI3K/Akt信号转导通路中pAkt的表达。Objective To observe the effects of Bushenhuoxue decoction on expression of p-Akt of PI3K/Akt signal transduction pathway in retinal ganglion cells（RGC）in rat model of chronic elevated intraocular pressure（EIOP）.Methods Thirty SD rats were randomly divided into 3 groups：control group,model group,treatment group,and EIOP models were established in meodel group and drug group.After the models established,the rats in treatment group were given the intragastric administration of Bushenhuoxue decoction,once per day for 8 weeks,and the control group and model group were given normal sodium.The intraocular pressure（IOP）before modeling and immediately,8 weeks after modeling were recorded.The number of RGC and retinal thickness were observed under the light microscope,the retinal ultrastructure was observed under the electron microscope,and the expression of pAkt in retina was detected by immunohistochemistry.Results Compared with immediately after modeling,IOP in treatment group at 8 weeks after modeling decreased,the difference was statistically significant（P〈0.01）.At 8 weeks after modeling,RGC number in treatment group and model group were lower than that in control group,the differences were statistically significant（all P〈0.05）;The difference in retinal thickness between control group and treatment group was not statistically significant（P〉0.05）,but the difference between control group and model group was statistically significant（P〈0.01）;RGC number and retinal thickness in treatment group were better than those in model group,the differences were statistically significant（all P〈0.01）.Under the electron microscope,the RGC ultrastructure injured obviously in model group,and the treatment group was improved.Immunohistochemical analysis of p-Akt expression in the retina showed that there were statistical differences in the total area,average optical density,integral optical density of p-Akt positive staining between treatment group and model group（all P〈0.01）,and the ave

关 键 词：补肾活血中药 视网膜神经节细胞 PI3K/Akt信号通路 P-AKT 青光眼 大鼠慢性高眼压模型 

分 类 号：R775[医药卫生—眼科]