γ-聚谷氨酸生产菌株的常压室温等离子体诱变选育  

Mutation Breeding of ?-Polyglutamic Acid Producing Strain by Atmospheric and Room Temperature Plasma

作　　者：陈双喜[1] 张二超[1] 张乐乐[1] 夏啟浩 

机构地区：[1]河南大学生命科学学院生物工程研究所,河南开封475004

出　　处：《中国医药工业杂志》2015年第9期960-963,共4页Chinese Journal of Pharmaceuticals

基　　金：河南省教育厅科学技术研究重点项目(14A416007)

摘　　要：γ-聚谷氨酸产生菌枯草芽孢杆菌(Bacillus subtilis)HD11经常压室温等离子体诱变处理,以不产生脂肽作为筛选标准之一,获得1株高产菌株HNCL1266,其γ-聚谷氨酸摇瓶发酵产量为26 g/L,较菌株HD11提高了30%,且遗传稳定。在5 L发酵罐上,添加0.2 g/L的泡敌可有效抑制泡沫的产生,γ-聚谷氨酸产量达到30 g/L。γ-Polyglutamic acid producing strain Bacillus subtilis HD11 was treated by atmospheric and room temperature plasma mutation, so as not to produce lipopeptides as one of the screening criteria. One mutant named HNCL1266 was obtained with the yield of γ-polyglutamic acid in shaking flask of 26 g/L, which was 30 % higher than that of HD11. The mutant strain showed high hereditary stability. In a 5 L fermentor, foams were suppressed effectively by addition of 0.2 g/L defoamer GPE. The yield of γ-polyglutamic acid reached to 30 g/L.

关 键 词：Γ-聚谷氨酸 常压室温等离子体 诱变 脂肽 选育 枯草芽孢杆菌 

分 类 号：TQ927[轻工技术与工程—发酵工程]