虎源细小病毒的分离鉴定及其VP2基因分析  

Isolation, identification and VP2 gene analysis of parvovirus from tiger

作　　者：刘永相[1] 李秀云 刘鑫[1] 蒋艳妹 郭冬春[1] 田进[1] 仇铮[1] 邹希明[1,2] 曲连东[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [2]北方森林动物园,黑龙江哈尔滨150332

出　　处：《中国预防兽医学报》2015年第9期721-724,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室开放基金项目(SKLVBF201404);中央级公益性科研院所基本科研业务费专项资金项目(0302014014)

摘　　要：为研究虎源细小病毒的分子遗传特征,本研究从东北虎腹泻粪便样品中分离到一株虎源细小病毒(HRB-T2014)。对该分离株的VP2基因进行克隆测序分析,结果表明,HRB-T2014与虎源细小病毒FJ405225和HT-374的相似性最高,达到99.9%,而与犬源细小病毒相似性最低,仅为98.1%。VP2基因核苷酸序列系统发育树分析结果显示,HRB-T2014与虎源中国分离株位于同一进化分支,并与猫和貂细小病毒共处于猫细小病毒分支,而与犬细小病毒处于不同的进化分支。以上结果表明,HRB-T2014与猫源细小病毒具有共同的遗传起源。To analysis the molecular and genetic characteristics of parvovirus originated from tigers, a virus was isolated from Siberian tiger diarrhea stool. The isolate was identified as parvovirus by PCR and electron microscope examination, designated HRB-T2014 , The nucleotide sequence analysis of VP2 gene showed that the isolate shared the highest similarity （99.9%） with tiger parvovirus FJ405225 and HT-374, and only 98.1% similarity with the canine parvovirus. The phylogenetic tree analysis of VP2 gene showed that the isolate and the FJ405225 and HT-374 were classified into the same branches with the feline and mink parvovirus, but located the different branches with canine parvovirus. This study suggested that the tiger parvovirus of HRB-T2014 and feline parvovirus originated from the common genetic ancestors.

关 键 词：虎 细小病毒 分离鉴定 VP2基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]