得力生通过调节血管蛋白抑制肝癌SMMC-7721细胞增殖并促凋亡  

Delisheng injection induced anti-proliferative and pro-apoptotic effects on SMMC-7721 cells through regulating angiogenic proteins

作　　者：孙海凤[1,2] 黎明[3] 赵征 郭亚焕 杨广宁 薛丽[2] 雷宝霞 南克俊[4] 

机构地区：[1]陕西省肿瘤医院,陕西西安710061 [2]西安交通大学医学部,陕西西安710061 [3]西安交通大学医学部第一附属医院心血管外科,陕西西安710061 [4]西安交通大学医学部第一附属医院肿瘤内科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2015年第5期702-706,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省自然科学基础研究计划项目(No.2014JM4183)~~

摘　　要：目的探讨得力生注射液对肝癌SMMC-7721细胞的作用机制。方法用不同浓度的得力生作用肝癌SMMC-7721细胞后,MTT方法检测肝癌SMMC-7721细胞不同时间(24、48、72h)的增殖情况,流式细胞技术检测细胞凋亡及细胞周期,免疫细胞化学方法检测肝癌SMMC-7721细胞中血管内皮生长因子(vascular endothelial growth factor,VEGF)、骨桥蛋白(osteopontin,OPN)和内皮抑素(endostadin,ENS)的表达。结果得力生作用于肝癌SMMC-7721细胞后,随着作用时间的延长和药物浓度的增加,细胞增殖逐渐减少,细胞凋亡逐渐增加,而对正常肝细胞HL-7702的作用很轻微。得力生将肝癌SMMC-7721细胞主要阻滞在G1期,且VEGF和OPN表达下调,ENS表达上调。结论得力生抑制肝癌SMMC-7721细胞增殖及诱导凋亡,呈现时间和浓度依赖效应,并可能通过调节血管蛋白而实现,这为得力生治疗肝癌提供了理论依据。Objective To investigate the possible anti-cancer mechanisms of Delisheng injection on hepatocellular carcinoma（HCC）cell line SMMC-7721.Methods Different concentration of Delisheng injection was administered on HCC cell line SMMC-7721.The cancer cell proliferation was measured by MTT assay.The apoptosis and cell cycle distribution were analyzed by flow cytometry.The expressions of vascular endothelial growth factor（VEGF）, osteopontin（OPN） and endostadin（ENS） in SMMC7721 cells were detected by immunocytochemistry staining.Results Delisheng injection treatment resulted in a significant decrease in cell proliferation and induced apoptotic cell death with the increase of treatment time and drug concentration.Additionally,Delisheng inhibited the proliferation of HCC SMMC-7721 cells strongly and the viability of normal liver HL-7702 cells slightly.Delisheng primarily arrested SMMC-7721 cells at the G1 phase of the cell cycle.Immunocytochemistry staining showed that VEGF and OPN expressions were downregulated whereas ENS expression was upregulated.Conclusion Delisheng inhibits the proliferation of HCC SMMC-7721 cells and induces their apoptosis in dose-and time-dependent manners.This effect may be realized by regulating angiogenic proteins,which provides experimental evidence for the treatment of HCC with Delisheng.

关 键 词：得力生 凋亡 细胞周期 肝癌 血管内皮生长因子(VEGF) 骨桥蛋白(OPN) 内皮抑素(ENS) 

分 类 号：R735.7[医药卫生—肿瘤]