肿瘤相关成纤维细胞在垂体腺瘤侵袭性行为中的作用研究  

The Role of Cancer-associated Fibroblasts in Invasive Behavior of Pituitary Adenoma

作　　者：钟传洪[1,2] 陶蓓[1] 吴瑶[2] 彭里磊[1] 周培志[2] 陈礼刚 姜曙[2] 

机构地区：[1]泸州医学院附属医院神经外科,泸州646000 [2]四川大学华西医院神经外科,成都610041

出　　处：《四川大学学报（医学版）》2015年第5期673-678,共6页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技厅科技支撑计划项目(No.2014SZ0019-9;No.2015SZ0120)资助

摘　　要：目的初步研究肿瘤相关成纤维细胞在垂体腺瘤侵袭性行为中的作用。方法依据术前磁共振(MRI)检查和术中观察,收集典型侵袭性(IPA组,n=7)和非侵袭性(nIPA组,n=8)垂体腺瘤新鲜组织,进行原代培养肿瘤相关成纤维细胞,并用肌动蛋白α(α-SMA)染色鉴定;利用Transwell迁移和侵袭性试验比较两组细胞的迁移和侵袭能力,并用免疫细胞化学、Western blot比较两组肿瘤相关成纤维细胞α-SMA、基质金属蛋白酶(MMP)-9的表达差异。结果新鲜垂体腺瘤组织中培养出α-SMA阳性的肿瘤相关成纤维细胞,两组细胞的迁移能力无明显差异,侵袭能力IPA组强于nIPA组(P=0.010);IPA组细胞MMP-9表达高于nIPA组(P=0.025),α-SMA的表达在两组之间差异无统计学意义。结论肿瘤相关成纤维细胞可能通过分泌更多的MMP-9增强自身侵袭能力,为肿瘤细胞侵袭周围组织提供条件。Objective To determine the role of cancer associated fibroblasts （CAFs）in the invasive behavior of pituitary adenoma. Methods Pituitary adenoma tissues were divided into invasive group （IPA） and non-invasive group （nIPA） according to pre-operative MRI and observations during surgery. Those tissues were cultured and CAFs were identified through a smooth muscle actin （α-SMA）. The migratory and invasive ability of CAFs was tested with transwell migration and invasion assay. The expressions of α-SMA and matrix metalloproteinase （MMP）-9 from CAFs were determined by immunocytochemistry and Western blot. Results All cultured CAFs expressed α-SMA. No significant difference in migratory ability of CAFs was found between the IPA and nlPA tissues; however, CAFs from the IPA tissues had stronger invasive ability than those from the nlPA tissues （P= 0. 010）. Higher levels of MMP-9 expression were found in group IPA as compared with nlPA （P= 0. 025）. No significant difference in the expression of α-SMA was found between the two groups. Conclusion CAFs may promote invasive behavior by secreting more MMP-9, which may play a part in the invasive behavior of pituitary adenomas.

关 键 词：肿瘤相关成纤维细胞 垂体腺瘤 侵袭性 MMP-9 

分 类 号：R736.4[医药卫生—肿瘤]