透明颤菌血红蛋白与耐低氧抗生素生产菌株构建  

Vitreoscilla Hemoglobin and Construction of the Hypoxia-tolerant Antibiotic-producing Strains

作　　者：赵亚兰[1] 徐春燕[1] 马悦[1] 苏建宇[1] 

机构地区：[1]宁夏大学生命科学学院西部特色生物资源保护与利用教育部重点实验室,中国宁夏银川750021

出　　处：《生命科学研究》2015年第4期347-352,共6页Life Science Research

摘　　要：透明颤菌血红蛋白(Vitreoscilla hemoglobin,VHb)是由透明颤菌属细菌产生的氧结合蛋白。通过引入透明颤菌血红蛋白基因(vgb)从降低需氧角度定向改造抗生素生产菌是近年来研究的热点。vgb在宿主菌中的异源表达能有效提高宿主菌在低氧环境下的代谢效率,促进代谢产物积累,基于这一特性,采用基因工程育种策略构建具有低氧耐受性的生产菌种,将有效降低抗生素生产成本。简述了近年来VHb的研究进展,重点总结了耐低氧抗生素生产菌株构建的研究及其应用现状,并对其在抗生素工业生产中的应用前景进行展望。The Vitreoscilla hemoglobin（VHb） from the bacterium Vitreoscilla sp. is an oxygen-binding protein. In recent years, via the introduction of the Vitreoscilla hemoglobin gene（vgb） to the host bacteria, VHb is attracting great attentions due to its potential in directional transformation of antibiotic-producing strains to hypoxia-tolerant recombinants. Under hypoxic conditions, both the metabolic efficiency and antibiotics yields can be increased though the expression of vgb in the host bacteria. Thus, the construction of hypoxiatolerant recombinants by gene engineering can reduce effectively the cost of antibiotics production. Firstly,the progress of VHb in recent years is briefly introduced, and then the construction of hypoxia tolerant antibiotic-producing strains is mainly summarized. Finally, the application prospects in antibiotics produc-tion are proposed.

关 键 词：透明颤菌血红蛋白 耐低氧菌株 菌株构建 抗生素 微生物发酵 

分 类 号：Q815[生物学—生物工程]