不同生物状态白色念珠菌对口腔上皮细胞的黏附能力及ALS mRNA表达  

Adhesion ability of Candida albicans with different biological states for oral epithelial cells and its ALS mRNA expression

作　　者：张辉 叶美花 俞诚波 张蓓蓓 蔡敏秋 许红苗 

机构地区：[1]浙江省温岭市第一人民医院口腔科,浙江温岭317500

出　　处：《中国医药导报》2015年第26期16-19,共4页China Medical Herald

基　　金：浙江省温岭市科技计划项目(2012WLCA0062)

摘　　要：目的观察不同生物状态白色念珠菌对口腔上皮细胞的黏附能力及ALS m RNA表达,以期揭示口腔白色念球菌感染机制。方法将白色念珠菌3683、SC5314、3630与来源于50名健康志愿者的口腔上皮细胞混合培养,采用革兰阳性染色观察白色念珠菌的黏附能力,采用荧光定量RT-PCR法检测白色念珠菌3683、SC5314、3630中ALS2及ALS3 m RNA表达情况。采用SPSS 15.0统计学软件进行数据分析。结果黏附实验结果显示,3株白色念珠菌均可黏附于口腔上皮细胞,且菌株3683黏附数量明显多于菌株SC5314和菌株3630,统计学比较显示,差异有统计学意义(P<0.05),而菌株SC5314和菌株3630黏附数量比较,差异无统计学意义(P>0.05)。荧光定量RT-PCR结果显示,白色念珠菌3683、SC5314、3630中均能检测到ALS2及ALS3 m RNA表达,其中,菌株3683ALS2及ALS3 m RNA表达水平均高于菌株SC5314和菌株3630,统计学比较显示,差异有统计学意义(P<0.05);菌株3630 ALS2及ALS3 m RNA表达水平均高于菌株SC5314,但两者比较,差异无统计学意义(P>0.05)。结论不同生物状态白色念珠菌的口腔上皮细胞黏附能力不同,菌株黏附能力的强弱可能与其ALS2及ALS3基因情况表达相关。Objective To observe the adhesion ability of Candida albicans with different biological states for oral ep-ithelial cells and its ALS mRNA expression, in order to reveal the mechanism of oral Candida albicans infection. Methods Candida albicans 3683, SC5314, 3630 and oral epithelial cells from 50 cases of healthy volunteers were mixed cultivation. Gram positive staining was used to observe the adhesion ability of Candida albicans. Candida albi-cans 3683, SC5314, 3630 ALS2 and ALS3 mRNA expressions were detected by fluorescent quantitation RT-PCR method. SPSS 15.0 statistical software was used for data analysis. Results Adhesion experiment results showed that Candida albicans 3683, SC5314, 3630 could stick to oral epithelial cells. Adhesion level of Candida albicans 3683 was higher than that of Candida albicans SC5314 and 3630, the differences were statistically significant （P〈0.05）. The ad-hesion level between Candida albicans SC5314 and 3630 had no statistically significant difference （P〉0.05）. Fluores-cent quantitation RT-PCR results showed that Candida albicans 3683, SC5314, 3630 could express ALS2 and ALS3 mRNA. The ALS2 and ALS3 mRNA expressions in Candida albicans 3683 were higher than those of Candida albicans SC5314 and 3630, the differences were statistically significant （P〈0.05）. The ALS2 and ALS3 mRNA expressions be-tween Candida albicans SC5314 and 3630 had no statistically significant difference （P 〉 0.05）. Conclusion Candida albicans with different biological states have different adhesion abilities for oral epithelial cells, which can be related with the expressions of ALS2 and ALS3 mRNA.

关 键 词：白色念珠菌 口腔上皮细胞 黏附能力 基因表达 

分 类 号：R781[医药卫生—口腔医学]