小鼠胚胎干细胞分泌因子对前列腺癌细胞作用的体外研究  

Effects of factors secreted by mouse embryonic stem cells on prostate cancer cells in vitro

作　　者：翟红运[1] 张登禄[1] 王光杰[1] 孔峰[2] 程广辉[2] 赵升田[1] 

机构地区：[1]山东大学第二医院泌尿外科,山东济南250033 [2]山东大学第二医院中心实验室,山东济南250033

出　　处：《山东大学学报（医学版）》2015年第9期8-12,23,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81272815);泰山学者特聘教授科研基金

摘　　要：目的研究小鼠胚胎干细胞(ESC)分泌因子对前列腺癌细胞RM-1生物学特性的影响,并探讨其作用机制。方法采用Transwell小室在体外建立ESC联合小鼠胚胎成纤维细胞(MEF)与RM-1细胞的非接触式共培养体系作为实验干预(实验组),同时建立MEF与RM-1细胞的共培养体系作为阴性对照(对照组)。共培养72 h后,检测实验组与对照组RM-1细胞的增殖水平,比较两组细胞的形态学、细胞周期、凋亡水平、迁移与侵袭能力的差异。结果与对照组比较,实验组RM-1细胞的形态表现为排布稀疏,分裂减少,死亡增多,部分细胞呈现凋亡迹象;实验组RM-1细胞增殖减慢(P<0.01),细胞周期存在G1→S期阻滞且凋亡增加,以晚期凋亡为主(P均<0.01);实验组RM-1细胞的迁移和侵袭能力减弱(P均<0.05)。结论 ESC分泌因子可通过阻滞RM-1细胞周期、促进其凋亡使RM-1细胞的增殖减慢,同时减弱其迁移与侵袭能力而发挥抗肿瘤作用。Objective To study the effects of factors secreted by mouse embryonic stem cells （ESC） on the biological characteristics of prostate cancer cells RM-1 and to explore the mechanism. Methods A non-contact co-culture system was established with Transwell chambers in vitro. The chambers connecting ESC combined with mouse embryonic fibroblasts （MEF） and RM-1 cells served as experimental group, while the chambers connecting MEF and RM-1 cells were negative controls. After cells were co-cultured for 72 h, the proliferation of RM-1 cells in the two groups were measured. Differences of RM-1 cells in cell morphology, cell cycle, apoptotic level, migration and invasion ability be- tween the two groups were observed. Results The RM-1 cells in experimental group were loosely arranged with less cell division and more cell death, and some cells showed signs of apoptosis. The RM-1 cells in experimental group pro- liferated more slowly （P 〈 0.01 ）, with cell cycle arrest between phase G1 and S in addition with increased apoptosis, es- pecially late stage apoptosis （both P 〈0.01 ）. The RM-1 cells in experimental group showed weaker migration and invasion ability （both P 〈 0.05 ）. Conclusion The factors secreted by ESC exhibited anti-tumor effects. They inhibited RM- 1 cell proliferation by arresting cell cycle, increasing cell apoptosis, and weakening cell migration and invasion ability.

关 键 词：胚胎干细胞 细胞环境 分泌因子 肿瘤 前列腺癌细胞RM-1 小鼠 

分 类 号：R737.25[医药卫生—肿瘤]