轮状病毒-肠道腺病毒荧光纳米颗粒联检试纸检测效能测评  

Evaluation of the detection performance of rotavirus-enteric adenovirus fluorescent nanoparticles combined test strip

作　　者：李琛[1] 兰巧芬 廖焕金 李尚姝 叶玲[1] 盛慧英[1] 潘庆军[1] 

机构地区：[1]广东医学院附属医院,湛江524001

出　　处：《中华实验和临床病毒学杂志》2015年第4期368-370,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金（81202346）;广东省卫生厅科研基金（A2014481）.

摘　　要：目的评估轮状病毒-肠道腺病毒抗原荧光纳米颗粒快速联检试纸条的灵敏度和特异性。方法以培养的轮状病毒、肠道腺病毒、以及其他肠道病原体，还有140例具有胃肠道感染症状患者的粪便标本，分别用轮状病毒-肠道腺病毒抗原荧光纳米颗粒快速联检试纸条和轮状病毒-腺病毒抗原二合一彩色微球法检测试纸条检测。结果轮状病毒-肠道腺病毒抗原荧光纳米颗粒快速联检试纸条的灵敏度显著高于轮状病毒-腺病毒抗原二合一彩色微球法检测试纸条；对常见的柯萨奇病毒（A16）、埃可病毒（30型）和肠道病毒（EV71）无交叉反应。结论轮状病毒-肠道腺病毒抗原的荧光纳米颗粒快速联检试纸条灵敏度显著高于彩色微球法快速检测试纸条，特异性良好。Objective To evaluate the sensitivity and specificity of rotavirus and enteric adenovirus antigen fluorescent nanoparticles rapid combined detection test strip. Methods The specimen including rotavirus, enteric adenovirus, and other enteric pathogens and 140 cases of patients with symptoms of gastrointestinal infection. The performance was analyzed after fluorescent nanoparticles rapid combined detection test strip and coloredmicrospheres rapid detection test strips were used to detect the same sample. Result The sensitivity of fluorescent nanoparticles rapid combined detection test strip is significantly higher than coloredmicrospheres rapid test strip and no specific reaction with Coxsachievirus （type A16）, ECHO virus （type 30） and enterovirus （type 71 ）. Conclusion The sensitivity of fluorescent nanoparticles rapid combined detection test strips is significantly higher than coloredmicrospheres rapid detection test strip, and the specificity is good.

关 键 词：腺病毒科 轮状病毒属 荧光纳米颗粒 彩色微球 敏感性与特异性 

分 类 号：R446.6[医药卫生—诊断学]