层粘连蛋白与Ⅳ型胶原在宫颈原位癌及微小浸润性癌鉴别诊断中的应用  

作　　者：李岱娟 岳良 孙永红[2] 王晓敏[2] 舒丽莎[3] 

机构地区：[1]乌兰察布市中心医院妇科,内蒙古乌兰察布012000 [2]潍坊医学院附属医院病理科,山东潍坊261000 [3]河北北方学院第一附属医院妇产科,河北张家口075000

出　　处：《实用妇科内分泌电子杂志》2015年第6期84-85,共2页Electronic Journal of Practical Gynecological Endocrinology

基　　金：国家科技部十一五重点支撑项目;妇科疾病现代治疗规范化研究(2008BAI57B00)

摘　　要：目的探讨层粘连蛋白(Laminin)与Ⅳ型胶原蛋白(CollagenⅣ)在宫颈原位癌与微小浸润癌鉴别诊断中的意义。方法应用免疫组化检测Laminin与CollagenⅣ在宫颈原位癌、微小浸润癌及浸润癌中的表达情况。结果 Laminin与CollagenⅣ的表达基本一致,两者在正常宫颈组织基底膜中呈完整连续性;原位癌中基底膜多数是连续性的,但着色深度不规则;微小浸润癌组基底膜呈不连续性,浸润癌组基底膜无着色,完全缺如。结论 Laminin与CollagenⅣ的表达不仅对原位癌与微小浸润癌的鉴别诊断有帮助,而且有助于预测原位癌发展为浸润癌。Objective To investigate the significance of laminin and collagen type IV in differential diagnosis between cervical carcinoma in situ and micro invasive carcinoma. Methods Immunohistochemistry was performed to determine the expression laminin and collagen protein in cervical carcinoma in situ, microinvasive carcinoma and invasive carcinoma. Results Expressions of laminin and collagen IV located on the basement membrane continuously in normal cervical tissue; their expression is uninterrupted in most the basement membrane of ductal carcinoma in situ, but the color intensity is irregular; in microinvasive carcinoma group, the colouration of basement membrane was discontinuous; basement membrane staining was completely absent cervical cancer group. Conclusion The expression of laminin and collagen IV could not only help to make diagnosis between carcinoma in situ and micro invasive carcinoma, but also could worked as a forecaster to predict the risk of developing invasive cancer

关 键 词：层粘连蛋白 Ⅳ型胶原蛋白 宫颈原位癌 微小性浸润癌 鉴别诊断 

分 类 号：R737.33[医药卫生—肿瘤]