七氟醚对小鼠肺癌细胞HP A及Fascin表达的影响  

Effects of sevoflurane on expression of heparanase and fascin in lung carcinoma cells of mice

作　　者：梁桦[1] 文先杰[1] 黄翔[1] 徐枫[1] 张涛[1] 杨承祥[1] 

机构地区：[1]佛山市第一人民医院麻醉科,528000

出　　处：《中华麻醉学杂志》2015年第5期577-579,共3页Chinese Journal of Anesthesiology

基　　金：广东省医学科研基金资助项目（B2013372）

摘　　要：目的：评价七氟醚对小鼠肺癌细胞乙酰肝素酶（ HPA）及Fascin表达的影响。方法小鼠Lewis肺癌细胞接种于培养板，培养24 h后，采用随机数字表法分为4组（ n＝18）：对照组（ C组）、1％七氟醚组（ Sev1组）、2％七氟醚组（ Sev2组）和3％七氟醚组（ Sev3组）。 C组不接受七氟醚处理，Sev1-3组细胞分别用1％、2％和3％七氟醚处理4 h，继续培养24 h。采用Transwell法检测侵袭细胞数，细胞划痕实验检测细胞迁移率；采用Western blot法检测HPA和Fascin表达。结果与C组比较，Sev1-3组侵袭细胞数和细胞迁移率依次降低，HPA和Fascin表达依次下调（ P ＜0．05）。结论七氟醚抑制小鼠肺癌细胞转移能力的机制与下调HPA和Fascin表达有关。Objective To evaluate the effects of sevoflurane on the expression of heparanase （ HPA） and fascin in lung carcinoma cells of mice. Methods Mouse LLC cells were inoculated in the culture plate. After being cultured for 24 h, the cells were equally and randomly divided into 4 groups using a random number table： control group （ group CC） , 1% sevoflurane group （ group Sev1 ） , 2% sevoflurane group （ group Sev2 ） , and 3% sevoflurane group （ group Sev3 ） . Cells in Sev1-3 groups were exposed to 1%, 2% and 3% sevoflurane, respectively, for 4 h, while cells in group CC were not exposed to sevoflurane, and all the cells were then cultured for another 24 h in an incubator. The invasion of cells was determined by Transwell invasion assay, and the invaded cells were counted. The migration of cells was determined by wound healing assay, and cell migration rates were calculated. The expression of HPA and fascin in cells was detected by Western blot. Results Compared with group CC, the number of invaded cells and cell migration rates were gradually decreased, and the expression of HPA and fascin was gradually down-regulated with increasing concentrations of sevoflurane in Sev1-3 groups. Conclusion The mechanism through which sevoflurane inhibits the metastasis of mouse lung carcinoma cells is associated with down-regulated expression of HPA and fascin.

关 键 词：麻醉药 吸入 肺肿瘤 肿瘤转移 肝素裂合酶 细胞运动 

分 类 号：R734.2[医药卫生—肿瘤] R614[医药卫生—临床医学]