β-环糊精修饰的荧光金纳米簇的合成及其在细胞成像上的应用（英文）  

Synthesis of β-Cyclodextrin Functionalized Fluorescent Gold Nanoclusters for Cellular Imaging

作　　者：孙敬华[1,2] 张卫[1,2] 谭彩萍[1,2] 计亮年[1,2] 毛宗万[1,2] 

机构地区：[1]生物无机与合成化学教育部重点实验室 [2]中山大学化学与化学工程学院,广州510275

出　　处：《无机化学学报》2015年第9期1913-1918,共6页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.21231007);国家重点基础研究发展计划(973计划)(No.2014CB845604)资助项目

摘　　要：通过一锅法水相合成出一种β-环糊精修饰的荧光金簇。该新型金簇具有红色荧光发射、长时间的分散稳定性和荧光稳定性。透射电镜观察其平均粒径为(1.40±0.32)nm。激光共聚焦显示金簇分散在细胞质中,荧光随金簇浓度增加而增强。细胞毒性实验证实该金簇具有良好的生物相容性。表面修饰的β-环糊精,也为金簇载药或者靶向功能化提供了更多可能性,从而进一步拓宽其在生物医学方面的应用。A new kind of highly fluorescent AuNCs functionalized with β-cyclodextrin （CD） was prepared by using one-pot aqueous reduction of gold precursor. Aqueous dispersion of these red AuNCs exhibits good long-term colloidal stability as well as fluorescent stability. The average diameter of AuNCs-CD is （1.40±0.32） nm as determined by transmission electron microscopy （TEM）. Confocal microscopic observation shows that AuNCs-CD can penetrate into ceils and mainly localize within the cytoplasm. In vitro cytotoxicity assay indicates that the AuNCs-CD displays very low cytotoxicity against human lung adenocarcinoma A549 ceils. Cyclodextrin modification is expected to make AuNCs more versatile for encapsulation of hydrophobic drugs and functionalization with targeting moieties, which will broaden the potential of AuNCs-CD for biomedical applications.

关 键 词：Β-环糊精 金纳米簇 细胞成像 

分 类 号：O614.123[理学—无机化学]