木瓜蛋白酶提取蛇皮胶原蛋白的工艺研究  

Research Process on the Extraction of Collagen with Papain from the Skin of Snake

作　　者：吴玉琼[1] 骆红霞[1] 胡永乐[1] 张静 温爱国 

机构地区：[1]武夷学院茶与食品学院,福建武夷山354300 [2]武夷山蛇类研究所,福建武夷山354300

出　　处：《武夷学院学报》2015年第6期26-30,共5页Journal of Wuyi University

基　　金：福建省科技厅重点项目(2012Y0063)

摘　　要：采用木瓜蛋白酶从人工养殖的蛇皮中提取胶原蛋白,研究了提取液的pH值、酶浓度、温度、固液比和提取时间对胶原蛋白提取率的影响。结果表明,木瓜蛋白酶提取蛇皮中胶原蛋白的最佳工艺为:提取温度30℃,提取剂柠檬酸,木瓜蛋白酶浓度3.00%、固液比1:10、pH值6、提取时间24 h,在此条件下胶原蛋白提取率为89.30%。Collagen of extraction was studied from the farmed snake skin.The extraction technology including pH, papain concentration, immersion temperature, solid-liquid, immersion time was discussed. The results indicated that the optimum extracted conditions are as fol- lows： at 30 ℃, in 0.1 mol/L citric acid buffer solution, the added enzyme dosage is 3.00% and the weight ratio of skin to solvent is 1 to 10, immersion time is 24 h. the recovery of the collagens was 89.30%.

关 键 词：蛇皮 胶原蛋白 木瓜蛋白酶 最佳参数 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]