NGX6基因转染对鼻咽癌细胞基因表达谱的影响  

Changes of Expression Profile Induced by NGX 6 Transfection in Nasopharyngeal Carcinoma Cells

作　　者：马健[1] 周洁[1] 李小玲[1] 龚佳蕾[2] 何显锋[2] 唐珂[1] 阳剑波[1] 胡赓熙[2] 李桂源[1] 

机构地区：[1]中南大学湘雅医学院肿瘤研究所,长沙410078 [2]中国科学院上海生物化学和细胞生物学研究所,上海200031

出　　处：《中国生物化学与分子生物学报》2002年第4期416-422,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家"973"基金资助项目 (No .G19980 5 10 0 8) ;"863"基金资助项目 (No.2 0 0 1AA2 2 10 3 1);国家自然科学基金资助项目 (No .3 0 0 0 0 0 96)

摘　　要：鼻咽癌是我国南方的常见肿瘤 .NGX6是新近克隆的定位于 9p2 1 2 2 ,在鼻咽癌组织中表达下调的基因 .初步的研究结果显示 ,NGX6基因转染鼻咽癌细胞HNE1能够延缓其生长速度 ,使肿瘤细胞更多地停留在G0 G1期 .为探索NGX6基因在鼻咽癌发病机制中的作用 ,建立了高表达NGX6的鼻咽癌细胞系 .利用包含 14 0 0 0个基因的cDNA微阵列分析了NGX6基因转染对HNE1细胞基因表达谱的影响 ,发现NGX6基因的转染能够上调p2 9、APC7、NEU1、RNasek6、αE catenin和TFⅡEα等基因的表达 ;同时也下调properdinP因子、G0S2、BAZ2B、ZHX1,OS4和PBX3等基因的表达 .研究结果提示 ,NGX6基因对鼻咽癌细胞的生物学行为的影响可能与它对一些细胞周期调控因子和转录调控因子的影响相关 .作为一种高通量的分析技术 。Nasopharyngeal carcinoma (NPC) is a common cancer among Southern Chinese. A novel gene entitled NGX 6 have been cloned, which derived from the tumor suppressor locus on chromosome 9p21 22 and down regulated in nasopharyngeal carcinoma. The preliminary functional analysis showed that the growth rate of NPC cells transfected with NGX 6 became slower than transfected with empty vector. The flow cytometry analysis showed that NGX 6 overexpression could arrest NPC cells in G 1 phase. To investigated the role of NGX6 in carcinoma proliferation in NPC, a NGX6 overexpression NPC cell line was established by gene transfection. Using cDNA microarray, the expression of 14 000 genes in cells overexpressing NGX6 in comparison with empty vector transfected cells was evaluated. The results indicated a panel of genes that could be modulated by NGX 6. NGX 6 overexpression downregulated genes, including properdin P factor, G0S2, BAZ 2B, ZHX1, OS 4, and PBX 3. In contrast, NGX 6 overexpression upregulated p 29, APC7, NEU1 , RNase k6, αE catenin, and TFⅡE α. The results indicated that NGX 6 overexpression might influence the growth rate of NPC cells by regulating some cell cycle regulators and transcription factors. As a high throughput analysis technique, cDNA miccroarray provided important clues for functional study of a novel gene.

关 键 词：NGX6 基因转染 鼻咽癌细胞 基因表达谱 CDNA微阵列 细胞周期 

分 类 号：R739.63[医药卫生—肿瘤]